Preparation and Field Evaluation of Mycoplasma bovisMembrane Vaccine in Egypt
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Most Trusted Research Journal Since Year 2012

ISSN: 2319-7064



Research Paper | Microbiology | Egypt | Volume 4 Issue 5, May 2015

Preparation and Field Evaluation of Mycoplasma bovisMembrane Vaccine in Egypt

Sabry Eissa

Aim To prepare M. bovis membrane vaccine and its evaluation in laboratory experiment and field study. Material and Methods In this study, a laboratory experiment was carried out to evaluate the safety and potency of the prepared M. bovis vaccine. A field study was applied on three bovine dairy farms at Fayoum and Behera Governorates for 6 months. Detection of M. bovis antibodies was measured using indirect ELISA. Results The laboratory experiment cleared that vaccinated & challenged group of rabbits with virulent field strain showed high immune response and the vaccine was able to protect rabbits against M. bovis infection. In the field study, a high immune response was detected in all vaccinated animals. Culture and PCR were negative in all vaccinated animals indicating that the vaccine provides a good protection against Mycoplasma bovis infection, while the contact animals gave positive results for isolation and PCR. Conclusion M. bovis membrane vaccine was prepared from local field isolate. In the laboratory experiment vaccinated rabbits were protected against challenge with M. bovis virulent field strain. In the field study, the vaccinated cows produced high immune response with negative culture and PCR, indicating that the prepared vaccine was able to protect the animals against M. bovis infection.

Keywords: Mycoplasma bovis, Membrane vaccine, dairy cows, indirect ELISA, PCR

Edition: Volume 4 Issue 5, May 2015

Pages: 660 - 664

Share this Article

How to Cite this Article?

Sabry Eissa, "Preparation and Field Evaluation of Mycoplasma bovisMembrane Vaccine in Egypt", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=SUB153048, Volume 4 Issue 5, May 2015, 660 - 664

55 PDF Views | 48 PDF Downloads

Download Article PDF



Similar Articles with Keyword 'indirect ELISA'

Research Paper, Microbiology, Tanzania, Volume 3 Issue 9, September 2014

Pages: 541 - 545

Seroprevalence of Paratuberculosis in Goats and Sheep in Arusha, Northern Tanzania

Fulgence Mpenda, Joram Buza

Share this Article

Research Paper, Microbiology, Egypt, Volume 4 Issue 5, May 2015

Pages: 660 - 664

Preparation and Field Evaluation of Mycoplasma bovisMembrane Vaccine in Egypt

Sabry Eissa

Share this Article

Similar Articles with Keyword 'PCR'

Research Paper, Microbiology, India, Volume 6 Issue 3, March 2017

Pages: 1928 - 1931

Detection of S. mutans and S. sobrinus from Dental Plaques Adhered on Zirconia, PFM and Ni-Cr Crown by RT-PCR

Shikha Gupta, Om Prakash, Dr. Raj Kumar Kalyan, Dr. Dev Kumar Verma, Dr. Jitendra Rao, Dr Chandkiram Gautam

Share this Article

Research Paper, Microbiology, India, Volume 6 Issue 8, August 2017

Pages: 2030 - 2034

Molecular Characterization of Azospirillum halopraeferens

K. Kanimozhi, A. Panneerselvam

Share this Article

Research Paper, Microbiology, India, Volume 4 Issue 7, July 2015

Pages: 2052 - 2054

mamAB Operon: Major Role in Biomineralisation within Magnetotactic Bacteria

Renu Singh, Sanjiv Kumar Maheshwari

Share this Article

Research Paper, Microbiology, Iraq, Volume 3 Issue 8, August 2014

Pages: 935 - 939

Using of astA and uidA Genes Characterization in Detection of Escherichia coli Prevalence from Human Gallstone

Abdalnabi J. Abid, Sama J. AL-zuwainy

Share this Article

Research Paper, Microbiology, Egypt, Volume 4 Issue 2, February 2015

Pages: 2082 - 2088

Serological and Molecular Typing of Avian Pathogenic E. Coli Originating from Outbreaks of Colibacillosis in Chicken Flocks

Sarah A. Yousef, Ammar A. M., Ahmed D. A.

Share this Article
Top