International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064




Downloads: 104

Research Paper | Microbiology | Egypt | Volume 4 Issue 2, February 2015


Serological and Molecular Typing of Avian Pathogenic E. Coli Originating from Outbreaks of Colibacillosis in Chicken Flocks

Sarah A. Yousef, Ammar A. M., Ahmed D. A.


Abstract: Avian pathogenic Escherichia coli (E. coli) is the etiological agents of colibacillosis, which is one of the principal causes of morbidity, mortality in poultry worldwide and responsible for significant economic losses to the poultry industry. Some outbreaks consistently associated with specific serotype, O111 causing mortality, septicemia and polyserositis with the chickens. Detection of Shiga toxin1, Shiga toxin 2 and Intimin genes that encode virulence factors, by multiplex PCR is an efficient method for identifying virulent strains of isolated E. coli. The objective of this study was serotyping of isolated E. coli, measurement of cytotoxicity of E. coli on Vero cell culture, detection of Shiga toxin1, Shiga toxin 2 and Intimin genes of isolated E. coli by using of Polymerase Chain Reaction. E. coli isolates was recovered from 510 samples with overall prevalence 75.2 %. The most prevalent E. coli serovar isolates recovered from different sources of poultry broiler farms were Untypable E. coli serovare, followed by O26, then O2, O124, O125, and O115. Isolated E. coli caused third degree of the cytopathic effect of Vero cell sheet. Out of 20 E. coli isolates recovered from various broiler samples, 5 isolates were positive for Shiga toxin 1 (Stx1) gene, 3 for Intimin gene, and 3 were positive for the Shiga toxin 2 (Stx2) gene.


Keywords: Escherichia coli, Shiga toxin 1, Shiga toxin 2, Intimin gene, broiler, Colibacillosis, Multiplex PCR


Edition: Volume 4 Issue 2, February 2015,


Pages: 2082 - 2088


How to Cite this Article?

Sarah A. Yousef, Ammar A. M., Ahmed D. A., "Serological and Molecular Typing of Avian Pathogenic E. Coli Originating from Outbreaks of Colibacillosis in Chicken Flocks", International Journal of Science and Research (IJSR), https://www.ijsr.net/get_abstract.php?paper_id=SUB151644, Volume 4 Issue 2, February 2015, 2082 - 2088

How to Share this Article?

Enter Your Email Address


Similar Articles with Keyword 'Escherichia coli'

Downloads: 26 | Monthly Hits: ⮙1

Research Paper, Microbiology, India, Volume 10 Issue 3, March 2021

Pages: 1461 - 1466

Biopreservation of Fruits (Tomato and Banana) Using Bacteriocin Produced by LACTOBACILLUS SPP.

B. Sivasankari, K. Narmatha, S. Ganesh

Share this Article

Downloads: 44

Research Paper, Microbiology, Nigeria, Volume 6 Issue 12, December 2017

Pages: 1484 - 1492

Phytochemical Determination and In Vitro Antimicrobial Activity of Crude Ethanolic Extract of Stem Bark of Boswellia dalzielii

Baha'uddeen Salisu Dandashire; Abdulkadir Magaji Magashi; Bashir Abdulkadir

Share this Article

Similar Articles with Keyword 'broiler'

Downloads: 137

Research Paper, Microbiology, India, Volume 8 Issue 3, March 2019

Pages: 619 - 625

Effective Role of Indigenous Microorganisms (IMOs) Farming Technology on Poultry Chickens Blood Profile

Somasekhar M, Sai Gopal DVR

Share this Article

Downloads: 140

Research Paper, Microbiology, Egypt, Volume 7 Issue 5, May 2018

Pages: 700 - 703

Susceptibility and Interleukins-6 Serum Level of Broilers Chickens Vaccinated with Baculovirus Expressed H5N1-ND after Experimental Infection with Virulent E. coli

Ismael Radwan, Ahmed Orabi

Share this Article

Similar Articles with Keyword 'Escherichia'

Downloads: 26 | Monthly Hits: ⮙1

Research Paper, Microbiology, India, Volume 10 Issue 3, March 2021

Pages: 1461 - 1466

Biopreservation of Fruits (Tomato and Banana) Using Bacteriocin Produced by LACTOBACILLUS SPP.

B. Sivasankari, K. Narmatha, S. Ganesh

Share this Article

Downloads: 44

Research Paper, Microbiology, Nigeria, Volume 6 Issue 12, December 2017

Pages: 1484 - 1492

Phytochemical Determination and In Vitro Antimicrobial Activity of Crude Ethanolic Extract of Stem Bark of Boswellia dalzielii

Baha'uddeen Salisu Dandashire; Abdulkadir Magaji Magashi; Bashir Abdulkadir

Share this Article

Similar Articles with Keyword 'coli'

Downloads: 139

Research Paper, Microbiology, Nigeria, Volume 9 Issue 6, June 2020

Pages: 742 - 745

Isolation of Common Nosocomialpathogens from Gate Pass Used in Aminu Kano Teaching Hospital and Bayero University Kano, Nigeria

Umar Adamu Hamza, Binta Muhammad Aminu, Muzammil Bashir Mustapha

Share this Article

Downloads: 1

Case Studies, Microbiology, India, Volume 10 Issue 11, November 2021

Pages: 1133 - 1134

Sphingobacterium Multivorum - A Case Report from a Spondylodiscitis Patient

Gargi Ghosh, Asad Riyaz, Anshul Agrawal

Share this Article

Similar Articles with Keyword 'toxin'

Downloads: 54

Research Paper, Microbiology, Ireland, Volume 10 Issue 2, February 2021

Pages: 667 - 673

Isolation and Identification of Soil Fungi from Ghemins, Libya

Latifa Abdel-Hafid Jwieli, Nadia Ali Elramli, Sanaa Rajab Othman, Naeema Matuoog

Share this Article

Downloads: 120 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Microbiology, India, Volume 4 Issue 5, May 2015

Pages: 712 - 716

Mycotoxins Contamination in Animal Food and Feed

Ashok K. Shukla

Share this Article

Similar Articles with Keyword '1'

Downloads: 139

Research Paper, Microbiology, Nigeria, Volume 9 Issue 6, June 2020

Pages: 742 - 745

Isolation of Common Nosocomialpathogens from Gate Pass Used in Aminu Kano Teaching Hospital and Bayero University Kano, Nigeria

Umar Adamu Hamza, Binta Muhammad Aminu, Muzammil Bashir Mustapha

Share this Article

Downloads: 0

Research Paper, Microbiology, Nigeria, Volume 10 Issue 2, February 2021

Pages: 1026 - 1029

Prevalence of Urinary Tract Infection among Age Groups in Bauchi Metropolis, Nigeria

S. Isma'il

Share this Article

Similar Articles with Keyword '2'

Downloads: 187 | Monthly Hits: ⮙1

Research Paper, Microbiology, India, Volume 6 Issue 1, January 2017

Pages: 296 - 299

Antibacterial behaviour of Common Winter Season Weeds on Human Pathogens in Doon Valley, Uttarakhand, India

Maneesha Singh, Rashmi Bist

Share this Article

Downloads: 139

Research Paper, Microbiology, Nigeria, Volume 9 Issue 6, June 2020

Pages: 742 - 745

Isolation of Common Nosocomialpathogens from Gate Pass Used in Aminu Kano Teaching Hospital and Bayero University Kano, Nigeria

Umar Adamu Hamza, Binta Muhammad Aminu, Muzammil Bashir Mustapha

Share this Article

Similar Articles with Keyword 'gene'

Downloads: 1 | Monthly Hits: ⮙1

Research Paper, Microbiology, India, Volume 10 Issue 11, November 2021

Pages: 663 - 667

The Degradation Pathway of 4-Chlorobenzoic Acid by Genetically Modified Strain of Pseudomonas aeruginosa

Ashok Kr. Singh

Share this Article

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Microbiology, India, Volume 11 Issue 1, January 2022

Pages: 677 - 686

Introduction of Successive Mutagenesis and Medium Optimisation for Enhanced Production of Ascomycin from Bacteria

Prabhakar Babu, Umesh Luthra, Ilma Majeed, Krishna Priya

Share this Article
Top