Research Paper | Microbiology | Ivory Coast | Volume 3 Issue 12, December 2014
Phosphate Solubilizing of Carob (Ceratonia siliqua L) Associative Bacteria Analyzed by Molecular Technique ARDRA
Ibrahim KONATE, Annick KOULIBALY, Ibourahema COULIBALY, El Bekkay BERRAHO
We aim use in this study, endophytic bacteria isolated from inside the roots (IRC) and epicotyls (IEC) of carob (Ceratonia siliqua L.) plants collected from different Moroccan regions and previously screened by Rep-PCR. 30 IRC and 4 IEC strains selected on the basis of Rep-PCR representative groups and 8 carob symbiotic bacteria (RCM) were tested for their solubilizing activities and antibiotic resistance and analyzed by PCR-RLFP technique. 70 % and 50 % of carob associative bacteria and RCM strains respectively were solubilizing phosphate in culture media with P solubilization index (SI) ranged from 1, 63 to 2, 13 for IRC and IEC and from 1, 25 to 1, 5 for RCM. Amplified ribosomal DNA analysis (ARDRA) of the isolates based on cleavage with four restriction enzymes yielded 25 genotypes with IRC and 3 and 5 differents profiles respectively with IEC and RCM. Dendrogram based on upon UPGMA analysis of ARDRA patterns showed a wide genetic diversity and divided the strains into six clusters at 50 % of similarity level. Two clusters revealed relatedness with the rhizobial reference strains used and four others clusters which contain mainly associative endophytic bacteria were more heterogeneous and very distinct from all the other strains.
Keywords: Associative bacteria, carob Ceratinia siliqua L, Phosphate solubilization, ARDRA
Edition: Volume 3 Issue 12, December 2014
Pages: 473 - 479
How to Cite this Article?
Ibrahim KONATE, Annick KOULIBALY, Ibourahema COULIBALY, El Bekkay BERRAHO, "Phosphate Solubilizing of Carob (Ceratonia siliqua L) Associative Bacteria Analyzed by Molecular Technique ARDRA", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=SUB14350, Volume 3 Issue 12, December 2014, 473 - 479
118 PDF Views | 103 PDF Downloads
Similar Articles with Keyword 'Phosphate solubilization'
Research Paper, Microbiology, Kenya, Volume 5 Issue 12, December 2016
Pages: 1030 - 1034Characterization of Multiple Plant Growth Promoting Traits of Serratia Spp. Associated With Vegetables Rhizospheric Soil
John N Ombusuro, K Ponmurugan
Research Paper, Microbiology, Ivory Coast, Volume 3 Issue 12, December 2014
Pages: 473 - 479Phosphate Solubilizing of Carob (Ceratonia siliqua L) Associative Bacteria Analyzed by Molecular Technique ARDRA
Ibrahim KONATE, Annick KOULIBALY, Ibourahema COULIBALY, El Bekkay BERRAHO
Research Paper, Microbiology, India, Volume 6 Issue 4, April 2017
Pages: 2086 - 2089Screening the Effect of Phosphate Solubilizing Bacteria on Black Gram (Vigna mungo L.)
Senthil Kumar.R, M. Bharathi
Research Paper, Microbiology, India, Volume 8 Issue 8, August 2019
Pages: 1683 - 1690Screening of Endophytic Actinomycetes for their Plant Growth Promoting Activity and for Biocontrol in Chilli (Capsicum annum) against Sclerotium Rolfsii
Khushboo, Navneet Kaur
Research Paper, Microbiology, India, Volume 4 Issue 8, August 2015
Pages: 400 - 404Comparative Study of Phosphate Solubilizing Bacteria from Paddy Field
Padmshree Patel, Pratibha Desai, Sharda Patil