Production of Polyhydroxybutyrate (biopolymer) by Bacillus tequilensis NCS-3 Isolated from Municipal Waste Areas of Silchar, Assam
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064


Amazon Sale


Research Paper | Microbiology | India | Volume 3 Issue 12, December 2014

Production of Polyhydroxybutyrate (biopolymer) by Bacillus tequilensis NCS-3 Isolated from Municipal Waste Areas of Silchar, Assam

Chandani N., Mazumder P. B., Bhattacharjee A.

Polyhydroxybutyrate (PHB) is a biodegradable polymer produced by many bacteria. It can be used as an alternative to petroleum-based commercial plastics as they can be easily degraded by the soil microorganisms. The present work focuses on screening a potential bacterium for PHB production. In this study, 50 bacterial colonies from the soil samples were isolated and screened for PHB production. They were grown in basally-defined M9 medium (DM9 medium) and screened for biosynthesis of PHB using Sudan Black B stain. 20 were found to be able to produce PHB and among them, isolate 3 showed highest productions of 1.0g/l PHB from 1.92g/l DCW (dry cell weight). Isolate 3 was characterized as Bacillus tequilensis NCS-3 based on 16S rRNA gene sequence. This isolate was selected to optimize its culture conditions using different carbon sources, nitrogen sources, pH and temperatures. After optimization, the isolate yielded maximum amount of 1.75g/l PHB from 2.01g/l DCW at pH 6 and incubation temperature of 300C using fructose and tryptone as carbon and nitrogen sources. PHB production increases from 52 % to 87 % after optimization, which indicates that Bacillus tequilensis NCS-3 was a potent PHB producer.

Keywords: Polyhydroxybutyrate PHB, Biopolymer, Bacillus tequilensis NCS-3, 16S rRNA, DM9 medium

Edition: Volume 3 Issue 12, December 2014

Pages: 198 - 203

Share this Article

How to Cite this Article?

Chandani N., Mazumder P. B., Bhattacharjee A., "Production of Polyhydroxybutyrate (biopolymer) by Bacillus tequilensis NCS-3 Isolated from Municipal Waste Areas of Silchar, Assam", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=SUB14261, Volume 3 Issue 12, December 2014, 198 - 203

62 PDF Views | 50 PDF Downloads

Download Article PDF


Amazon Sale


Similar Articles with Keyword 'Biopolymer'

Research Paper, Microbiology, India, Volume 3 Issue 12, December 2014

Pages: 198 - 203

Production of Polyhydroxybutyrate (biopolymer) by Bacillus tequilensis NCS-3 Isolated from Municipal Waste Areas of Silchar, Assam

Chandani N., Mazumder P. B., Bhattacharjee A.

Share this Article

Research Paper, Microbiology, India, Volume 4 Issue 10, October 2015

Pages: 1895 - 1901

Isolation and Culture Conditions Optimization For PHB Production by Pseudochrobactrum asaccharolyticum

Monika Sharma, Harish Kumar Dhingra

Share this Article

Similar Articles with Keyword '16S rRNA'

Research Paper, Microbiology, India, Volume 6 Issue 8, August 2017

Pages: 2030 - 2034

Molecular Characterization of Azospirillum halopraeferens

K. Kanimozhi, A. Panneerselvam

Share this Article

Research Paper, Microbiology, India, Volume 3 Issue 12, December 2014

Pages: 198 - 203

Production of Polyhydroxybutyrate (biopolymer) by Bacillus tequilensis NCS-3 Isolated from Municipal Waste Areas of Silchar, Assam

Chandani N., Mazumder P. B., Bhattacharjee A.

Share this Article

Research Paper, Microbiology, India, Volume 6 Issue 2, February 2017

Pages: 2007 - 2012

Isolation, Screening and Production of Biosurfactant by PSEUDOMONAS AEROGINOSA SD4 Using Various Hydrocarbon Sources

Abirami Sivasubramani, Radhathirumalaiarasu Selvaraj

Share this Article

Research Paper, Microbiology, India, Volume 4 Issue 1, January 2015

Pages: 2644 - 2650

Production of Indole Acetic Acid (IAA) by Stenotrophomonas Maltophilia BE25 Isolated from Roots of Banana (musa spp)

M. S. Ambawade, G.R. Pathade

Share this Article

Research Paper, Microbiology, India, Volume 4 Issue 2, February 2015

Pages: 1373 - 1379

Isolation of Antagonistic Actinomycetes SPS from Rhizosphere of BT Cotton

T. Sujatha, P. Siva Raagini

Share this Article
Top