International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Since Year 2012 | Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064




Downloads: 3 | Monthly Hits: ⮙1

Research Paper | Biomedical Sciences | Indonesia | Volume 10 Issue 8, August 2021


Allium Ascalonicum L. Ethanolic Extract Inhibited the Decrease of eNOS but did not Inhibit the Decrease of Spatial Memory in D-Galactose-Induced Balb/c Male Mice

Yunita [12] | Wimpie Pangkahila [21] | Ni Nyoman Ayu Dewi [2]


Abstract: Background: Currently, the focus of health services has shifted to maintaining cognitive function in old age. Impaired cognitive function can be caused by aging of blood vessels, characterized by decreased endothelial function and nitric oxide (NO). Shallots have been proven to contain flavonoids which have a neuroprotective effect by increasing cerebral blood flow. This study evaluates the effectiveness of the ethanolic extract of shallots in inhibiting the decrease of spatial memory and eNOS levels. Method: The randomized pre-posttest control group design was carried out on 26 healthy three-month-old Balb/C male mice with a 20-30 grams bodyweight, divided equally into two random groups. The control group was given 2 ml of aquadest orally, while the treatment group was given ethanolic extract of shallots at a dose of 16.8mg/20gBW/day dissolved in 2 mL of aquadest. After two hours of administration, both groups were induced with D-Galactose orally 0.3 mg/gr BW/day once a day. Treatments were given daily for four weeks. Measurement of eNOS levels and spatial memory using Morris Water Maze test was carried out before the intervention (pretest) and after (posttest). Comparative analysis was conducted to evaluate the differences between the two groups and a correlation test between eNOS levels and spatial memory was performed. Results: The pre-posttest comparison resultsof eNOS showed a significant decrease (P<0.05) in both groups, however the difference between posttest-pretest (? post-pretest) showed that the decrease was significantly higher in control compared to the treatment group (p=0.031).This indicates that shallot ethanolic extract was able to inhibit the decrease of eNOS level. The pre-posttest comparison results showed a significant decrease (P<0.05) in spatial memory and ? post-pretest did not differ (p>0.05) between control and treatment groups. This indicates shallot ethanolic extract did not inhibit the decrease of spatial memory. No significant correlation was foundbetween eNOS and spatial memory. Conclusion: Administration of oral ethanolic shallots extractinhibited the decreaseofeNOS level but did not inhibit the decrease of spatial memory. The potential of shallots needs to be studied furtherto get an effective period of administration and doses.


Keywords: Allium Ascalonicum L., D-Galactose, eNOS, Shallots, Spatial memory


Edition: Volume 10 Issue 8, August 2021,


Pages: 315 - 319


How to Cite this Article?

Yunita, Wimpie Pangkahila, Ni Nyoman Ayu Dewi, "Allium Ascalonicum L. Ethanolic Extract Inhibited the Decrease of eNOS but did not Inhibit the Decrease of Spatial Memory in D-Galactose-Induced Balb/c Male Mice", International Journal of Science and Research (IJSR), Volume 10 Issue 8, August 2021, pp. 315-319, https://www.ijsr.net/get_abstract.php?paper_id=SR21807170607

How to Share this Article?

Enter Your Email Address




Similar Articles with Keyword 'L'

Downloads: 139 | Monthly Hits: ⮙1

Comparative Studies, Biomedical Sciences, Sri Lanka, Volume 6 Issue 12, December 2017

Pages: 608 - 615

To Identify and Examine the Different Causes of Liver Disease in Sri Lanka

Fahim Aslam | Hashan Kulasena

Share this Article

Downloads: 0

Research Paper, Biomedical Sciences, Congo, Volume 10 Issue 8, August 2021

Pages: 645 - 648

Evolutionary Profile of Drepanocytic Patients Followed at the National Drepanocytosis Reference Centre (CNRDr) Congo Brazzaville

Edwige Paola Chancelle LOUANGA NANITELAMIO [2] | Serge Oscar MOKONO [2] | Chaldam Jesp?re MBANI | Olivia Galiba Atipo - Tsiba | Fabien Roch NIAMA [3] | Etienne MOKONDJIMOBE [4] | Donatien MOUKASSA [6] | Ange Antoine ABENA [4] | Alexis ELIRA DOKEKIAS [2]

Share this Article


Top