International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Since Year 2012 | Open Access | Double Blind Reviewed

ISSN: 2319-7064


Downloads: 76

Masters Thesis | Biomedical Sciences | Indonesia | Volume 10 Issue 2, February 2021


Oral Administration of Ethyl Acetate Extract of Sanrego (Lunasia amara Blanco) Wood Increased the Number of Leydig Cells and Testosterone Levels in Old Male Wistar Rats (Rattus norvegicus)

Florindo Cardoso Gomes | Wimpie Pangkahila [22] | I Gusti Made Aman [5]


Abstract: In older men, a decrease in various hormones such as testosterone occurs. It results in andropause. A decrease in testosterone levels due to aging is associated with a decrease in the number of Leydig cells. The efforts to increase the number of Leydig cells and testosterone levels is an Anti-Aging Medicine step. Ethyl acetate extract of Sanrego (Lunasia amara Blanco) wood contains alkaloids, flavonoids, tannins, steroids, antioxidants, terpenoids, polyphenols, and steroids. The aim of this study was to prove that oral administration of ethyl acetate extract of Sanrego (Lunasia amara Blanco) wood increase the number of Leydig cells and testosterone levels in old male Wistar rats (Rattus norvegicus). Methods: This study was true experimental research using randomized posttest-only control group design. Subjects were 36 rats (Rattus norvegicus), Wistar strain, male, aged 18 months and weighing 275 grams. Rats were divided into two groups, the control group was given aquabidest 2 ml as a placebo using intragastric forced-feeding once a day and the treatment group was given ethyl acetate extract of Sanrego Wood of 16.5 mg/275gr. BW of rats dissolved in 2 ml aquabidest using intragastric forced-feeding once a day. After 21 days of treatment, blood was drawn on the right or left medial canthus sinus orbitalis for the examination of testosterone levels (ELISA method) and testes were taken for examination of Leydig cell numbers (histopathological method).


Keywords: Sanrego wood ethyl acetate extract, Leydig cells, male Wistar rats


Edition: Volume 10 Issue 2, February 2021,


Pages: 131 - 135


How to Download this Article?

You Need to Register Your Email Address Before You Can Download the Article PDF


How to Cite this Article?

Florindo Cardoso Gomes, Wimpie Pangkahila, I Gusti Made Aman, "Oral Administration of Ethyl Acetate Extract of Sanrego (Lunasia amara Blanco) Wood Increased the Number of Leydig Cells and Testosterone Levels in Old Male Wistar Rats (Rattus norvegicus)", International Journal of Science and Research (IJSR), Volume 10 Issue 2, February 2021, pp. 131-135, https://www.ijsr.net/get_abstract.php?paper_id=SR21129204955

Similar Articles with Keyword 'Leydig cells'

Downloads: 5 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Biomedical Sciences, Indonesia, Volume 10 Issue 6, June 2021

Pages: 844 - 847

The Administration of Zinc Inhibited the Decrease of Leydig Cell and Testosterone Level in Male Wistar Rats (Rattus Norvegicus) that are Exposed to Electric Cigarette Smoke

Novita Indah Permatasari | Wimpie I. Pangkahila [3] | Luh Putu Indrayani Maker

Share this Article

Downloads: 44

Research Paper, Biomedical Sciences, Indonesia, Volume 9 Issue 3, March 2020

Pages: 1625 - 1629

Oral Administration of Cassia Alata L. Leaves Ethanol Extract Prevented the Decrease of Leydig Cell Number but Did Not Decrease of Testosterone Level in Male Wistar Rats (Rattus Norvegicus) Exposed to Cell Phone Radiation

Ni Made Dharmiyanti | Wimpie Pangkahila [22] | A.A.A.N. Susraini [5]

Share this Article
Top