International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064

Downloads: 61

Doctoral Thesis | Medical Microbiology | Iraq | Volume 10 Issue 2, February 2021

Molecular Assessment of Gene Expression for Interleukine-4 and Interleukin-17 among Patients with Human Bronchial Asthma by Conventional and Real Time q-PCR Techniques

Ihsan Edan Alsaimary, Falih Hmood Mezban

This study aimed to detect IL 4 and IL 17 by conventional and RT PCR. DNA-based techniques have been further simplified benefiting from the introduction of PCR the result of this estimation revealed that the amplified DNA (PCR product) was 710 bp for IL-4 (50 voltage for 1 hour) and the presence of IL-4 was 85 % from total samples, (PCR product) was 344 bp for IL-17 on agarose gel (1 %) The presence of IL-17 was 70 % from total samples. RT PCR IL-4 show peak between 84.5 85.2 and IL-17 peak between 86.5-87.5.

Keywords: conventional and Real Time q-PCR techniques, human bronchial asthma, interleukins

Edition: Volume 10 Issue 2, February 2021

Pages: 165 - 169

Share this Article

How to Cite this Article?

Ihsan Edan Alsaimary, Falih Hmood Mezban, "Molecular Assessment of Gene Expression for Interleukine-4 and Interleukin-17 among Patients with Human Bronchial Asthma by Conventional and Real Time q-PCR Techniques", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=SR21119160619, Volume 10 Issue 2, February 2021, 165 - 169

Enter Your Email Address


Similar Articles with Keyword 'conventional'

Downloads: 121

Research Paper, Medical Microbiology, Nigeria, Volume 8 Issue 7, July 2019

Pages: 1369 - 1373

Detection of Artemisinin Target Gene (PFATP6) in Plasmodium Falciparum Infections among Asymptomatic Patients Attending NDUTH, FMC and General Hospital in Bayelsa State

Oluwayemisi A. Olorode, Ken K. Ovienadu, Tolulope Alade

Share this Article

Downloads: 128

Research Paper, Medical Microbiology, Nigeria, Volume 8 Issue 3, March 2019

Pages: 635 - 639

Evaluation of Direct Nitrate Reductase Assay for Drug Susceptibility Testing of Mycobacterium tuberculosis in Resource - Poor Settings

Olubunmi Alaka, Anuoluwapo Alaka, Olamide Agboola, Augusta Onuoha

Share this Article

Similar Articles with Keyword 'Time'

Downloads: 4

Review Papers, Medical Microbiology, India, Volume 10 Issue 7, July 2021

Pages: 227 - 230

Novel Coronavirus (COVID-19) - A Review

Adil Raza

Share this Article

Downloads: 110

Research Paper, Medical Microbiology, India, Volume 8 Issue 9, September 2019

Pages: 1222 - 1224

Evaluation of Antibacterial Activity of Iron Oxide Nanoparticles against Staphylococcus aureus

Harini.S

Share this Article

Similar Articles with Keyword 'techniques'

Downloads: 92

Research Paper, Medical Microbiology, Afghanistan, Volume 9 Issue 7, July 2020

Pages: 1824 - 1829

Recent Diagnostic Techniques for COVID-19

Dr Mohibullah Salih

Share this Article

Downloads: 95

Research Paper, Medical Microbiology, India, Volume 9 Issue 5, May 2020

Pages: 1592 - 1595

Bacterial Profile and Antibiotic Susceptibility Pattern of Lower Respiratory Tract Infections in a Tertiary Care Hospital

Manoj Kumar, Nayannika Lakra, Ashok Kumar Sharma, Amber Prasad, Kumari Seema

Share this Article

Similar Articles with Keyword 'human'

Downloads: 1

Research Paper, Medical Microbiology, India, Volume 10 Issue 8, August 2021

Pages: 863 - 869

Antimicrobial Susceptibility Pattern of Multi Drug Resistant (MDR) Pseudomonas aeruginosa in a Tertiary Care Hospital from Central India

Manish Patidar, Dr. Naveen Dhingra

Share this Article

Downloads: 4

Review Papers, Medical Microbiology, India, Volume 10 Issue 7, July 2021

Pages: 227 - 230

Novel Coronavirus (COVID-19) - A Review

Adil Raza

Share this Article
Top