Molecular Assessment of Gene Expression for Interleukine-4 and Interleukin-17 among Patients with Human Bronchial Asthma by Conventional and Real Time q-PCR Techniques
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064

Doctoral Thesis | Medical Microbiology | Iraq | Volume 10 Issue 2, February 2021

Molecular Assessment of Gene Expression for Interleukine-4 and Interleukin-17 among Patients with Human Bronchial Asthma by Conventional and Real Time q-PCR Techniques

Ihsan Edan Alsaimary, Falih Hmood Mezban

This study aimed to detect IL 4 and IL 17 by conventional and RT PCR. DNA-based techniques have been further simplified benefiting from the introduction of PCR the result of this estimation revealed that the amplified DNA (PCR product) was 710 bp for IL-4 (50 voltage for 1 hour) and the presence of IL-4 was 85% from total samples, (PCR product) was 344 bp for IL-17 on agarose gel (1%) The presence of IL-17 was 70 % from total samples. RT PCR IL-4 show peak between 84.5 ? 85.2 and IL-17 peak between 86.5-87.5.

Keywords: conventional and Real Time q-PCR techniques, human bronchial asthma, interleukins

Edition: Volume 10 Issue 2, February 2021

Pages: 165 - 169

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Ihsan Edan Alsaimary, Falih Hmood Mezban, "Molecular Assessment of Gene Expression for Interleukine-4 and Interleukin-17 among Patients with Human Bronchial Asthma by Conventional and Real Time q-PCR Techniques", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=SR21119160619, Volume 10 Issue 2, February 2021, 165 - 169

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