International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064


Downloads: 78

Research Paper | Biotechnology | India | Volume 10 Issue 1, January 2021


Development of Drought Tolerant Transgenic Cotton through Agrobacterium Mediated Transformation

Sarosha Nasreen, J. Amudha, S. S. Pandey


Abstract: The growth and productivity of cotton (Gossypium hirsutum L. ) are adversely affected by abiotic stresses such as dehydration. Plants adaptation to these stresses is controlled by cascades of molecular networks, including the dehydration responsive element binding (DREB) transcription factors, which specifically interact with C-repeat/DRE (A/GCCGAC), play an important role in plant environmental stress tolerance by controlling the expression of many stress related genes. In this work, an optimized cotton regeneration system from shoot apices was developed. The hypocotyl explants were transformed with Agrobacterium tumefaciens strain LBA4404 harboring the recombinant binary vector containing the DREB gene and Neomycin phosphotransferase (nptII) gene as a selectable marker under the control of CaMV 35S promoter. New putative plantlets were subsequently regenerated and confirmed by Polymerase chain reaction and southern blot analysis.


Keywords: Abiotic stress, DREB, Gossypium, hirsutum, Stress tolerance, Transgenic plants


Edition: Volume 10 Issue 1, January 2021,


Pages: 323 - 330


How to Cite this Article?

Sarosha Nasreen, J. Amudha, S. S. Pandey, "Development of Drought Tolerant Transgenic Cotton through Agrobacterium Mediated Transformation", International Journal of Science and Research (IJSR), https://www.ijsr.net/get_abstract.php?paper_id=SR21105122143, Volume 10 Issue 1, January 2021, 323 - 330

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