Evaluation of Phytochemical Antioxidant Properties and Quantification of Thymoquinone in Nigella sativa L. Seed Extract
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Most Trusted Research Journal Since Year 2012

ISSN: 2319-7064



Research Paper | Botany | India | Volume 9 Issue 9, September 2020

Evaluation of Phytochemical Antioxidant Properties and Quantification of Thymoquinone in Nigella sativa L. Seed Extract

Saliya S. Bawani, D. Usha Anandhi

Herbal drugs have been considered to be a beneficial remedy for various diseases. They are considered to have fewer side effects compared to modern medicines. In the world of herbal medicine, Nigella sativa L (Ranunculaceae), is one such herb held in high esteem owing to its notable pharmacological efficacies like neuroprotective and antioxidative properties. The present study aims to analyse the polyphenolic content, and anti-oxidant properties, and to quantify Thymoquinone (TQ) in N.sativa extract. Ethanolic extract was screened for possible antioxidant activity against 1, 1-diphenyl-2-picrylhydrazyl (DPPH), Hydroxyl radical, Superoxide anion radical and Hydrogen peroxide. Further, quantification of TQ was performed by HPLC. The results confirmed that N. sativa extract possesses positive antioxidant properties, which can be attributed to the presence of bioactive compounds. Thus, this extract can be considered as an effective agent with high therapeutic value in preventing cellular damage. Exploration of these chemicals might lead to novel drug discovery which might be an effective remedy for neurological disorder.

Keywords: Nigella sativa, Antioxidant activity, Thymoquinone, HPLC, Ethanolic extract

Edition: Volume 9 Issue 9, September 2020

Pages: 228 - 234

Share this Article

How to Cite this Article?

Saliya S. Bawani, D. Usha Anandhi, "Evaluation of Phytochemical Antioxidant Properties and Quantification of Thymoquinone in Nigella sativa L. Seed Extract", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=SR20903232448, Volume 9 Issue 9, September 2020, 228 - 234

42 PDF Views | 30 PDF Downloads

Download Article PDF



Similar Articles with Keyword 'Nigella sativa'

Research Paper, Botany, India, Volume 9 Issue 9, September 2020

Pages: 228 - 234

Evaluation of Phytochemical Antioxidant Properties and Quantification of Thymoquinone in Nigella sativa L. Seed Extract

Saliya S. Bawani, D. Usha Anandhi

Share this Article

Research Paper, Botany, India, Volume 7 Issue 2, February 2018

Pages: 1568 - 1571

Studies on Seed Mycoflora of Nigella Sativa L during Pre Harvest, Post Harvest and Storage Conditions

Adeeba Anwar, A. R. Ansari

Share this Article

Similar Articles with Keyword 'Antioxidant activity'

Research Paper, Botany, India, Volume 9 Issue 9, September 2020

Pages: 228 - 234

Evaluation of Phytochemical Antioxidant Properties and Quantification of Thymoquinone in Nigella sativa L. Seed Extract

Saliya S. Bawani, D. Usha Anandhi

Share this Article

Research Paper, Botany, Malaysia, Volume 4 Issue 8, August 2015

Pages: 1244 - 1249

In Vitro Antioxidant Activity of Ethanolic Extract from Terminalia Catappa (L.) Leaves and Fruits: Effect of Fruit Ripening

Abdulaziz Rabiu Abdulkadir

Share this Article

Comparative Studies, Botany, India, Volume 6 Issue 1, January 2017

Pages: 1999 - 2003

Comparative Study on Anti Diabetic Property of Syzyium cumini, Aegle marmelos and Cocos nucifera through in vitro and in vivo Condition

Mani Priya.B, Subha Ranjani.S

Share this Article

Research Paper, Botany, India, Volume 4 Issue 4, April 2015

Pages: 2738 - 2741

Characterization of Brassinosteroid Isolated from Bacopa monnieri L. and their Free Radical Scavenging Activity

Sarita Tripathi, Priyanka Sharma

Share this Article

Research Paper, Botany, India, Volume 4 Issue 9, September 2015

Pages: 962 - 965

In Vitro Evaluation of Nitric Oxide Scavenging Activity of Guettarda speciosa Linn.

Revathi D., Rajeswari M.

Share this Article

Similar Articles with Keyword 'HPLC'

Research Paper, Botany, India, Volume 9 Issue 9, September 2020

Pages: 228 - 234

Evaluation of Phytochemical Antioxidant Properties and Quantification of Thymoquinone in Nigella sativa L. Seed Extract

Saliya S. Bawani, D. Usha Anandhi

Share this Article

Comparative Studies, Botany, India, Volume 6 Issue 1, January 2017

Pages: 1999 - 2003

Comparative Study on Anti Diabetic Property of Syzyium cumini, Aegle marmelos and Cocos nucifera through in vitro and in vivo Condition

Mani Priya.B, Subha Ranjani.S

Share this Article

Research Paper, Botany, India, Volume 7 Issue 11, November 2018

Pages: 1052 - 1054

RP-HPLC Analysis of Flavonoids in Marsilea Minuta L

Revathi.M, Catharin Sara.S

Share this Article

Similar Articles with Keyword 'Ethanolic extract'

Research Paper, Botany, India, Volume 9 Issue 9, September 2020

Pages: 228 - 234

Evaluation of Phytochemical Antioxidant Properties and Quantification of Thymoquinone in Nigella sativa L. Seed Extract

Saliya S. Bawani, D. Usha Anandhi

Share this Article

Research Paper, Botany, India, Volume 5 Issue 2, February 2016

Pages: 2127 - 2130

In-vitro Assays to Show the Antioxidant Potential of ?-sitosterol from Lawsonia inermis Leaves

Sanjukta Badhai, Durga P. Barik, Bairagi C. Mallick

Share this Article

Research Paper, Botany, India, Volume 5 Issue 12, December 2016

Pages: 1178 - 1182

Determination of Phytocompounds in Spermacose hispida Leaf Extract Using Gas Chromatography and Mass Spectroscopic Technique

P. Shanthy, M. Kandhasamy

Share this Article

Research Paper, Botany, India, Volume 3 Issue 10, October 2014

Pages: 1833 - 1837

Antibacterial Activity from Aerial Parts of Double Flower Variety of Tabernemontana Divaricata

C. Kalaimagal, G. Umamaheswari

Share this Article

Research Paper, Botany, Malaysia, Volume 4 Issue 8, August 2015

Pages: 1244 - 1249

In Vitro Antioxidant Activity of Ethanolic Extract from Terminalia Catappa (L.) Leaves and Fruits: Effect of Fruit Ripening

Abdulaziz Rabiu Abdulkadir

Share this Article
Top