International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Since Year 2012 | Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064




Downloads: 68

Research Paper | Dentistry | India | Volume 9 Issue 11, November 2020


An in Vitro Comparison of Antimicrobial Efficacy and Residual Antimicrobial Activity of Root Canal Irrigants Qmix, 3% Sodium Hypochlorite, 2% Chlorhexidine against Enterococcus Faecalis

S Bindu [2] | N Shubhashini | I B Geeta


Abstract: AIM: To evaluate the antimicrobial activity and the residual antimicrobial activity of Qmix, 3 % NaOCl and 2 % chlorhexidine (CHX) against E. faecalis in human root dentin in vitro. METHOD: Assessment of antimicrobial activity was done with a dentin disk put into 2 ml Eppendorf tube containing 200μl E. faecalis suspension in BHI broth. The tubes were incubated under anaerobic conditions at 37°C for 3 weeks. The different root canal irrigants of 200 μl each was put in each tubes. After 2 min incubation, the dentin blocks were removed and dried with absorbent paper. The biofilm biomass was measured by crystal violet (CV) staining and the absorbance measurement values were obtained by spectrophotometry at 600 nm. Assessment of residual antimicrobial activity of different groups was done by measuring the absorbance values at different intervals of 0 h, 24 h and 48 h by spectrophotometry at 600nm. RESULTS: Qmix showed highest antimicrobial activity followed by 3 % NaOCl and 2 % CHX against E. faecalis biofilm. The residual antimicrobial activities of the irrigants 2 % CHX showed maximum reduction in E. faecalis biofilm at 0 hr. CHX residual antimicrobial activity became significant at 48 h. Qmix showed reduction in E. faecalis biofilm at the end of 48 h NaOCl did not show any residual antimicrobial activity CONCLUSION: In conclusion, our study showed that 3 % NaOCl, 2 % CHX and Qmix could kill E. faecalis in matured biofilm. The antibacterial activity of QMix excelled among the irrigants included in the study and the residual antimicrobial activity of 2 % CHX was better than QMix at 24h and 48h respectively. Clinical Significance: Antibacterial activity and residual antimicrobial activity are important properties of root canal irrigants against persistent microorganisms like E. Faecalis


Keywords: Root canal irrigants, Enterococcus Faecalis, Sodium hypochlorite, Chlorhexidine, QMix root canal irrigant,


Edition: Volume 9 Issue 11, November 2020,


Pages: 750 - 753

An in Vitro Comparison of Antimicrobial Efficacy and Residual Antimicrobial Activity of Root Canal Irrigants Qmix, 3% Sodium Hypochlorite, 2% Chlorhexidine against Enterococcus Faecalis


How to Cite this Article?

S Bindu, N Shubhashini, I B Geeta, "An in Vitro Comparison of Antimicrobial Efficacy and Residual Antimicrobial Activity of Root Canal Irrigants Qmix, 3% Sodium Hypochlorite, 2% Chlorhexidine against Enterococcus Faecalis", International Journal of Science and Research (IJSR), https://www.ijsr.net/get_abstract.php?paper_id=SR201111002641, Volume 9 Issue 11, November 2020, 750 - 753, #ijsrnet

How to Share this Article?

Enter Your Email Address




Similar Articles with Keyword 'Chlorhexidine'

Downloads: 1

Comparative Studies, Dentistry, India, Volume 10 Issue 5, May 2021

Pages: 578 - 595

A Comparison of the Efficacy of Turmeric Gel (2%) as Local Drug Delivery Agent with Scaling and Root Planing in the Treatment of Chronic Periodontitis: A Clinical Study

Rosiline Savio Vadakkan [2]

Share this Article

Downloads: 90

Research Paper, Dentistry, India, Volume 9 Issue 6, June 2020

Pages: 1615 - 1620

Comparative Evaluation of Anti- Plaque Efficacy of Commercially Available Herbal and 0.2% Chlorhexidine Gluconate Mouthwash: A Randomized Clinical Trial

Suresh Peddengatagari | Suma Priyanka Chenchu | Krishna Naimisha Punugu | Shanthi Velpula

Share this Article


Top