Identification of SSR Markers for Testing of Hybridity and Seed Genetic Purity in Maize (Zea Mays L.)
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064



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Research Paper | Genetics Science | India | Volume 3 Issue 10, October 2014

Identification of SSR Markers for Testing of Hybridity and Seed Genetic Purity in Maize (Zea Mays L.)

Sudharani M, Rao PS, Subba Rao LV

The present investigation was undertaken to identify specific marker associated with the maize hybrid DMH-117 and its parental lines, hybrid purity testing as well as identify the off-types from the seed lot. The hybrid DHM-117 could be clearly identified by using the SSR loci Umc1600 based on the banding pattern resolved on agarose gel (3 %). The complementary banding pattern of the male and female parents made a way to identify the hybrid. The SSR loci Umc1600 amplified a specific allele size of 180bp in seed parent (BML-6) and 195bp was specific to pollen parent BML-7. These two bands of allele size 180bp and 195bp were found in hybrid DHM-117, there by confirming that this hybrid was produced from the cross combination of BML-6 and BML-7.

Keywords: Genetic purity, Maize hybrids, SSR markers

Edition: Volume 3 Issue 10, October 2014

Pages: 92 - 98

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How to Cite this Article?

Sudharani M, Rao PS, Subba Rao LV, "Identification of SSR Markers for Testing of Hybridity and Seed Genetic Purity in Maize (Zea Mays L.)", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=OCT144, Volume 3 Issue 10, October 2014, 92 - 98

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