Interleukin-17 Role on the Occurrence of Plasma Leakage Dengue Hemorrhagic Fever Through Cytokine Proinflamasi Activation: (Study of in-vivo and in-vitro macrophage culture and cell endothelia)
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
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Research Paper | Medicine Science | Indonesia | Volume 3 Issue 11, November 2014

Interleukin-17 Role on the Occurrence of Plasma Leakage Dengue Hemorrhagic Fever Through Cytokine Proinflamasi Activation: (Study of in-vivo and in-vitro macrophage culture and cell endothelia)

Edi Hartoyo, Rasjad Indra, Edi Widajanto, Sri Rezeki Hadinegoro

INTERLEUKIN-17 ROLE ON THE OCCURRENCE OF PLASMA LEAKAGE DENGUE HEMORRHAGIC FEVER THROUGH CYTOKINE PROINFLAMASI ACTIVATION (Study of in-vivo and in-vitro macrophage culture and cell endothelia) Edi Hartoyo1, Rasjad Indra2, Edi Widajanto3, Sri Rezeki Hadinegoro4 1Children Health departement, Medical Faculty of Lambung Mangkurat University Banjarmasin 2 Laboratory of Molecular physiologies, Medical Faculty of Brawijaya University Malang 3Laboratory of clinic pathology, RSUD Saiful Anwar, Medical Faculty of Brawijaya Malang University 4 Children Health study, Medical Faculty of Indonesia University, Jakarta s Background: The role of IL-17 in virus infection has been evaluated, but there isnt data about its role in dengue hemorrhagic fever. The research purpose step 1 was to measure Th17 and IL-17 level on Dengue Haemorrhagic Fever shock and non shock. The second was to know the exposure effect of IL-17 recombinant on macrophage culture so it secreted TNF-, MMP-2 and IL-8. The third was to know the plasma leakage on cell endothelial culture which was exposed by using activated macrophage. Methods: The measurement of interleukin 17 was done by using ELISA method, Th17 intracell by flowcytrometry, while TNF, MMP-2 and IL-8 was measured by ELISA method. Albumin concentration was evaluated by spectroscopy. Results: Theres significant difference on Th17 and IL-17 level on DHF non shock case compared by DHF shock with p

Keywords: Plasma leakage, Dengue Hemorrhagic Fever DHF, IL-17, cytokine proinflamasi

Edition: Volume 3 Issue 11, November 2014

Pages: 1711 - 1717

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Edi Hartoyo, Rasjad Indra, Edi Widajanto, Sri Rezeki Hadinegoro, "Interleukin-17 Role on the Occurrence of Plasma Leakage Dengue Hemorrhagic Fever Through Cytokine Proinflamasi Activation: (Study of in-vivo and in-vitro macrophage culture and cell endothelia)", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=OCT141263, Volume 3 Issue 11, November 2014, 1711 - 1717

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