International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064




Downloads: 101

Case Studies | Botany | Iraq | Volume 5 Issue 4, April 2016


In vitro Shoot Regeneration and Antioxidant Enzymes Activities of Thevetianeriifolia Juss

Siham A.A. Salim, Basheer A. Al-Alwani, Rihab E. Kadhim


Abstract: Thevetia neriifolia is one of the important plants because it's a good source of natural compounds that have therapeutically effects. The main aim of this study is to investigate the effect of plant growth regulators on in vitro callus induction and regeneration of shoots from it, as well as the estimation of antioxidant enzymes activities during these processes. Callus was induced from internodal explants by culturing them on MS medium contained BA ( 0.0, 1.0 or 2.0 mg/L) and IBA ( 0.0, 1.0 or 2.0 mg/L), then shoot regeneration from callus was occurred. Results showed that the combinations of 1.0 mg/L BA+ 1.0 mg/L IBA, 1.0 mg/L BA+ 2.0 mg/L IBA, 2.0 mg/L BA+ 1.0 mg/L IBA and 2.0 mg/L BA+ 2.0 mg/L IBA were the best for callus induction (100%). The highest regeneration frequency (100%) and large mean of shoots regeneration ( 7.29) were achieved in MS medium supplemented with 1.0 mg/L BA + 0.0 mg/L IBA. A positive relationship was observed between regeneration capacity and antioxidant enzymes activities in which the highest levels of CAT and SOD activities were reached during the stages of shoots regeneration from callus.


Keywords: Antioxidant enzymes, callus, Thevetia neriifolia, in vitro, shoot regeneration


Edition: Volume 5 Issue 4, April 2016,


Pages: 2234 - 2238


How to Cite this Article?

Siham A.A. Salim, Basheer A. Al-Alwani, Rihab E. Kadhim, "In vitro Shoot Regeneration and Antioxidant Enzymes Activities of Thevetianeriifolia Juss", International Journal of Science and Research (IJSR), https://www.ijsr.net/get_abstract.php?paper_id=NOV162787, Volume 5 Issue 4, April 2016, 2234 - 2238

How to Share this Article?

Enter Your Email Address


Similar Articles with Keyword 'Antioxidant enzymes'

Downloads: 109

Research Paper, Botany, India, Volume 3 Issue 6, June 2014

Pages: 1546 - 1549

Biochemical Mode of Resistance to Environmentally Toxic Heavy Metal Lead in two Species of Alternanthera

Devi Chinmayee M, Mahesh B, Mini I, Swapna T S

Share this Article

Downloads: 109

Research Paper, Botany, India, Volume 6 Issue 3, March 2017

Pages: 1775 - 1780

Effect of Polyalthia Longifolia Leaves on Liver Carcinogenesis

Ambika.V, Sagayagiri.R

Share this Article

Similar Articles with Keyword 'callus'

Downloads: 34 | Monthly Hits: ⮙1

Research Paper, Botany, India, Volume 10 Issue 3, March 2021

Pages: 976 - 978

In Vitro Callus Induction studies in Salvadora persica L.

Pramod Fuke, Sangeeta Ahuja, Narayan B Pandhure

Share this Article

Downloads: 83

Review Papers, Botany, India, Volume 9 Issue 11, November 2020

Pages: 1489 - 1492

A Review of Hormone: AUXIN

Ranna Chaudhary, Ruby Patel

Share this Article

Similar Articles with Keyword 'vitro'

Downloads: 1

Research Paper, Botany, India, Volume 10 Issue 10, October 2021

Pages: 523 - 525

In vitro Multiplication of Terminalia Bellerica (Gaertn.) Roxb.

Pallavi Tandlepatil, Sangeeta Ahuja

Share this Article

Downloads: 33

Research Paper, Botany, India, Volume 10 Issue 3, March 2021

Pages: 951 - 953

In Vitro Multiplication of Salvadora Persica L.

Pramod Fuke, Sangeeta Ahuja, Narayan B Pandhure

Share this Article

Similar Articles with Keyword 'shoot regeneration'

Downloads: 33

Research Paper, Botany, India, Volume 10 Issue 3, March 2021

Pages: 951 - 953

In Vitro Multiplication of Salvadora Persica L.

Pramod Fuke, Sangeeta Ahuja, Narayan B Pandhure

Share this Article

Downloads: 34 | Monthly Hits: ⮙1

Research Paper, Botany, India, Volume 10 Issue 3, March 2021

Pages: 976 - 978

In Vitro Callus Induction studies in Salvadora persica L.

Pramod Fuke, Sangeeta Ahuja, Narayan B Pandhure

Share this Article

Similar Articles with Keyword 'Antioxidant'

Downloads: 150

Informative Article, Botany, India, Volume 6 Issue 1, January 2017

Pages: 2053 - 2055

Phytochemical Screening and Nutrient Analysis in Pulp Extract of Cucurbita Maxima

Keerthika, Mani Priya

Share this Article

Downloads: 108

Research Paper, Botany, India, Volume 4 Issue 7, July 2015

Pages: 2133 - 2134

Phenolics and Petal Senescence in Uncut Flower Petals of Tithonia rotundifolia Blake

Ruby Patel, Archana Mankad

Share this Article

Similar Articles with Keyword 'enzymes'

Downloads: 101

Research Paper, Botany, India, Volume 4 Issue 5, May 2015

Pages: 1880 - 1885

Effective Polycyclic Aromatic Hydrocarbons Degrading Rhizobacteria with PGP Traits from Plants Growing on Fly Ash in the Premises of Thermal Power Station?Kothagudem, Telangana State, India

M. Poornachander Rao, A.B. Rajithasri, T. Ashokvardhan, K. Satyaprasad

Share this Article

Downloads: 109

Research Paper, Botany, India, Volume 3 Issue 6, June 2014

Pages: 1546 - 1549

Biochemical Mode of Resistance to Environmentally Toxic Heavy Metal Lead in two Species of Alternanthera

Devi Chinmayee M, Mahesh B, Mini I, Swapna T S

Share this Article
Top