International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Since Year 2012 | Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064




Downloads: 127

Research Paper | Botany | India | Volume 5 Issue 1, January 2016


Callus Culture and in vitro Shoot Regeneration from Stem Segments of Endemic and Medicinally Important Caralluma lasiantha (Wight) N.E.Br.

V. Aruna [3] | C. Kiranmai | T. Pullaiah [3]


Abstract: Present protocol describes callus induction from stem segments and indirect organogenesis of Caralluma lasiantha, an endemic and traditional medicinal plant. Among different media used, MS medium was selected for callus studies as well as for organogenesis based on percentage of response, fresh and dry weight of the callus. Optimum amount of callus was obtained from MS + 2, 4-D 3mg/l + BA 0.1mg/l. Maximum response (60 %) of regeneration from callus was observed on MS + BA 1 mg/l + 2iP 0.5 mg/l. In vitro rooting was carried on strength MS medium supplemented with NAA 0.1mg/l for better survival of plantlets. In vitro regenerated plantlets were successfully established in soil with 70 % survival rate.


Keywords: Callus culture, Caralluma lasiantha, organogenesis, in vitro rooting, in vitro propagation


Edition: Volume 5 Issue 1, January 2016,


Pages: 903 - 908

Callus Culture and in vitro Shoot Regeneration from Stem Segments of Endemic and Medicinally Important Caralluma lasiantha (Wight) N.E.Br.


How to Cite this Article?

V. Aruna, C. Kiranmai, T. Pullaiah, "Callus Culture and in vitro Shoot Regeneration from Stem Segments of Endemic and Medicinally Important Caralluma lasiantha (Wight) N.E.Br.", International Journal of Science and Research (IJSR), https://www.ijsr.net/get_abstract.php?paper_id=NOV152756, Volume 5 Issue 1, January 2016, 903 - 908, #ijsrnet

How to Share this Article?

Enter Your Email Address




Similar Articles with Keyword 'Callus culture'

Downloads: 34

Research Paper, Botany, India, Volume 10 Issue 3, March 2021

Pages: 976 - 978

In Vitro Callus Induction studies in Salvadora persica L.

Pramod Fuke [2] | Sangeeta Ahuja [3] | Narayan B Pandhure [2]

Share this Article

Downloads: 114

Research Paper, Botany, India, Volume 4 Issue 4, April 2015

Pages: 3013 - 3015

Callus culture of Spilanthes paniculata (DC) Jansen- An Aromatic Medicinal plant

Nathar Varsha N. | Yatoo Ghulam M.

Share this Article


Top