International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064




Downloads: 122

Research Paper | Pharmacology Science | India | Volume 5 Issue 1, January 2016


Attenuation of Ischaimia Reperfusion Induced Myocardial Cell Injury in Rat Heart by Calpastatin

Rashmi Arora


Abstract: Myocardial ischaemia reperfusion is associated with activation of intracellular death proteases known as calpains. Isolated rat heart was subjected to 30 min ischaemia followed by reperfusion for 120min. The effect of calpain inhibitor calpastatin in induced myocardial injury assessed in terms of infract size measured macroscopically, release of LDH and CK have been investigated. Agarose gel electrophoresis was used to assess the DNA fragmentation. TUNEL staining was done to investigate the apoptotic index. Calpastatin has attenuated ischaemia reperfusion induced increase in LDH, CK, myocardial infarct size, DNA smearing and apoptotic index. It may be concluded that inhibition of intracellular death proteases prevented ischaemia reperfusion induced myocardial cell injury in terms of apoptosis and necrosis.


Keywords: Calpain, Calpastatin, Myocardial injury, Mitochondrial damage, Rat heart


Edition: Volume 5 Issue 1, January 2016,


Pages: 546 - 549


How to Cite this Article?

Rashmi Arora, "Attenuation of Ischaimia Reperfusion Induced Myocardial Cell Injury in Rat Heart by Calpastatin", International Journal of Science and Research (IJSR), https://www.ijsr.net/get_abstract.php?paper_id=NOV152716, Volume 5 Issue 1, January 2016, 546 - 549

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