Characteristics of New Primary Oral Cancer (Sp-C1) Cell and Antitumor Activity of Oligonucleotide p27Kip1 Sense on a Sp-C1 Cell
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064



Downloads: 137

Research Paper | Medicine Science | Indonesia | Volume 5 Issue 1, January 2016

Characteristics of New Primary Oral Cancer (Sp-C1) Cell and Antitumor Activity of Oligonucleotide p27Kip1 Sense on a Sp-C1 Cell

Supriatno, Sartari Entin Yuletnawati

New primary oral cancer (Sp-C1) cell has some characteristics of cell growth, expression of anti-apoptosis protein and positive regulatory protein of cell cycle. Characteristics of Sp-C1 cell is very important to facilitate the researchers using an in vitro model of oral cancer cells. In the present study, p27Kip1 sense (p27 S), p27Kip1 antisense (p27AS) and p27Kip1 neo (p27 neo) were transfected into Sp-C1 cells. The aim of study was to introduce the new primary oral cancer cell with their characteristics and to examine the antitumor activity of oligonucleotide p27 S on Sp-C1 cell in vitro. Isolasion of Sp-C1 cell was derived from cervical lymph node tissue of tongue cancer patient. As a final step of isolation, new fibroblast and primary oral cancer cells were cultured and stocked. The efficiency of p27 S and p27 AS transfection in cell growth inhibition test was confirmed by MTT [3- (4, 5-dimethythiazol-2-yl) -2, 5-diphenyltetrazolium bromide] assay. Colorimetric assay caspase-3 and caspase-9 were carried-out to know the apoptosis induction. The efficiency of oligonucleotide p27 transfection at protein level was confirmed by Western blotting analysis. Results of study revealed characteristics of Sp-C1 was relative fast in cell growth and positive expression of Skp2, -tubulin, CDK-2, cyclin-E, and maspin protein. Furthermore, Sp-C1 cell growth was markedly suppressed by p27 S. Also, p27 S-treated cell induced apoptosis characterized by an increase proteolytic activities of caspase-3 and -9. Up-regulation of p27Kip1 protein was detected in Sp-C1 p27 S. In conclusion, Sp-C1 cells had a certain characteristics and appropriate for an in vitro study model of oral cancer. p27 S had a strong antitumor activity on Sp-C1 cell. Targeting this molecule could represent a promising new therapeutic approach for this type of cancer.

Keywords: cell characteristic, Sp-C1cell, p27 S, antitumor activity

Edition: Volume 5 Issue 1, January 2016

Pages: 429 - 434

Share this Article

How to Cite this Article?

Supriatno, Sartari Entin Yuletnawati, "Characteristics of New Primary Oral Cancer (Sp-C1) Cell and Antitumor Activity of Oligonucleotide p27Kip1 Sense on a Sp-C1 Cell", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=NOV152655, Volume 5 Issue 1, January 2016, 429 - 434

Enter Your Email Address




Similar Articles with Keyword 'antitumor activity'

Downloads: 111

Research Paper, Medicine Science, Indonesia, Volume 5 Issue 1, January 2016

Pages: 1619 - 1623

High Antitumor Activity of Ethanolic Extracts of Papua's Ant Nest Plant (Myrmecodia tuberosa) on an Oral Carcinoma (KB) Cell Line

Sartari Entin Yuletnawati, Edi Meiyanto, Dewi Agustina

Share this Article

Downloads: 115

Research Paper, Medicine Science, Egypt, Volume 4 Issue 2, February 2015

Pages: 2170 - 2176

Potential Toxicity of Egyptian Ashwagandha: Significance for their Therapeutic Bioactivity and Anticancer Properties

Wafaa Abdallah Ahmed, Mohamed A., Nasser E. A., Doaa E.

Share this Article

Similar Articles with Keyword 'cell'

Downloads: 134

Research Paper, Medicine Science, India, Volume 3 Issue 6, June 2014

Pages: 1591 - 1598

Increasing Prevalence of Chronic Obstructive Pulmonary Disease;Tuberculosis; Lung Cancer and Rising Environmental Oestrogen

Elizabeth JeyaVardhini Samuel, Nagarajan Natarajan

Share this Article

Downloads: 82

Research Paper, Medicine Science, Indonesia, Volume 4 Issue 12, December 2015

Pages: 1639 - 1644

Up-Regulation of S-Phase Kinase Associated Protein-2 Antisense Induces Cell Growth and Migration Chemotactic Suppression and Apoptosis in a Malignant Oral Burkitts Lymphoma Cells

Supriatno, Sartari Entin Yuletnawati

Share this Article

Similar Articles with Keyword 'characteristic'

Downloads: 50

M.Tech / M.E / PhD Thesis, Medicine Science, India, Volume 6 Issue 8, August 2017

Pages: 618 - 622

Alcohol and Tobacco Use among the HIV/TB Co-Infected Individuals: A Case of Nepal

Bhim Bahadur Subba 'Phudong'; Dr. Nirmal Rimal

Share this Article

Downloads: 99

Research Paper, Medicine Science, France, Volume 6 Issue 12, December 2017

Pages: 1498 - 1504

Horizontal Extrapolation of Wind Speed Distribution Using Neural Network for Wind Resource Assessment

Nihad AGHBALOU, Abderafi CHARKI, Saida RAHALI ELAZZOUZI, Kamal REKLAOUI

Share this Article



Top