International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064


Downloads: 88

Research Paper | Veterinary Sciences | India | Volume 4 Issue 11, November 2015


Isolation Studies of Leptospira in Andhra Pradesh

Dr. D. Rani Prameela; Dr. D. Sreenivasulu; Dr. P. Vijayachari; Dr. Ch. Srilatha; Dr. S. Vijaya Lakshmi


Abstract: Isolation attempts were carried out between November 2006 and May 2010. Samples collected from clinically suspected cases of cattle (26), sheep (42), dogs (13), pigs (15), humans (53), stagnated water (10) in Rice fields and from rats (299) were inoculated in to EMJH liquid medium with tween- 80, antibiotics and 5- fluorouracil. A total of 17 isolates were recovered, from sheep (5), pigs (4), humans (2), rice field water (1) and from rats (5). The isolates were purified and maintained in EMJH liquid medium and as well as semi solid medium. The Physio-chemical characterization of isolates at 13oC, growth in the presence of 8 - Azaguanine, Lipase activity and animal inoculation studies revealed the pathogenicity of the isolates. The isolates were identified using Dark field microscopy, Fontanas staining, characteristic Dingers phenomenon and by histopathological studies.


Keywords: Leptospira, EMJH medium, Dark field microscopy, Fontana's staining, Physico chemical characterisation and Histopathology.


Edition: Volume 4 Issue 11, November 2015,


Pages: 1620 - 1625


How to Cite this Article?

Dr. D. Rani Prameela; Dr. D. Sreenivasulu; Dr. P. Vijayachari; Dr. Ch. Srilatha; Dr. S. Vijaya Lakshmi, "Isolation Studies of Leptospira in Andhra Pradesh", International Journal of Science and Research (IJSR), https://www.ijsr.net/get_abstract.php?paper_id=NOV151534, Volume 4 Issue 11, November 2015, 1620 - 1625

How to Share this Article?

Enter Your Email Address


Top