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Research Paper | Microbiology | India | Volume 10 Issue 11, November 2021
The Degradation Pathway of 4-Chlorobenzoic Acid by Genetically Modified Strain of Pseudomonas aeruginosa
Abstract: Pseudomonas aeruginosa a genetically modified strain degrading 4-chlorobenzoic acid was isolated from soil. This bacterium could degrade 15 mM of 4-chlorobenzoic acid rapidly with stoichiometric formation of chloride ion. The metabolites such as catechol and cis, cis-muconic acid was detected in the grown medium. Further, ring cleavage enzyme catechol 1, 2-dioxygenase was detected and isolated in crude cell extract. Thus, the biodegradation pathway of 4-chlorobenzoic acid by this bacterium was proposed involve the catechol as intermediate compound and further this converted into cis, cis-muconic acid by the process ortho ring cleavage pathway.
Keywords: 4-chlorobenzoic, Pseudomonas aeruginosa, 4-chlorocatechol, catechol, cis, cis-muconic acid
Edition: Volume 10 Issue 11, November 2021,
Pages: 663 - 667
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