Research Paper | Biology | Nigeria | Volume 2 Issue 2, February 2013
Occurrence and Antibiogram of Escherichia Coli O157:H7 in Locally Fermented Milk (Nono) Sold Under Market Conditions in Nasarawa State, Nigeria
C. R. Reuben, E.C. Okolocha, M. Bello, H. Tanimu
Escherichia coli O157:H7 is a newly emerging pathogen frequently associated with the consumption of food of bovine origin. Severe and life threatening human diseases caused by E. coli O157:H7 strains have been reported throughout the world. This present study evaluated the occurrence of E. coli O157:H7 in locally-fermented milk (nono) sold under market conditions in Nasarawa State, Nigeria and the patterns of their antibiotic susceptibility. A total of 420 nono samples were purchased across Nasarawa State. The samples were bacteriologically analyzed in the laboratory for the presence of E. coli O157:H7 by means of cultural techniques (enrichment on modified tryptone soy broth and selective plating on Cefixime-Tellurite Sorbitol Mac-Conkey Agar), biochemical (Microbact 12E) and serological assays. Oxoid diagnostic kit; latex (R30959601) was used to confirm E. coli O157:H7 respectively. Confirmed isolates were further subjected to antimicrobial susceptibility test using the Agar disc diffusion technique. The results of the study showed that out of 420 nono samples examined, 19 (4.5%) were contaminated with E. coli O157:H7. Among samples examined, the highest occurrence rate (5.7%) was recorded in samples obtained from Akwanga, Wamba and Doma Local Government Areas while Lafia and Keffi had the least occurrence rate (2.9%). With respect to the senatorial zones, Nasarawa North had the highest occurrence rate of 5.7% while the Southern zone had the least (3.6%). There was no significant difference (P>0.05) in the occurrence of E. coli O157:H7 isolated from nono samples with respect to the various Local Government Areas. Antibiotics susceptibility profile showed that all the isolates were resistant to multiple antibiotics, except ciprofloxacin and gentamicin, resulting in nine different resistance patterns. All the nineteen (100%) of the isolates were resistant to penicillin and tetracycline, 18 (94.7%) to erythromycin, 16 (84.2%) to amoxicillin, oxacillin and sulphamethoxazole/trimethoprim, 13 (68.4%) to chloramphenicol and 8 (42.1%) to streptomycin. 15 (78.9%) and 17 (89.5%) of the isolates were sensitive to ciprofloxacin and gentamicin. The predominant antimicrobial resistance pattern was penicillin-tetracycline-chloramphenicol-amoxycilin-erythromycin-oxacillin-sulphamethoxazole/ trimethoprim with the occurrence rate of 36.8% from samples obtained from Wamba, Doma, Kokona and Keffi. Nono consumption has potential health risks to consumers in Nasarawa State, hence proper hygiene in the process-line and marketing of nono is recommended. The multiple antimicrobial resistance exhibited by E. coli O157:H7 strains in this study is an indication of possible antibiotic abuse.
Keywords: E. coli O157:H7, emerging, locally fermented milk, Antibiotics, resistance
Edition: Volume 2 Issue 2, February 2013
Pages: 591 - 598
How to Cite this Article?
C. R. Reuben, E.C. Okolocha, M. Bello, H. Tanimu, "Occurrence and Antibiogram of Escherichia Coli O157:H7 in Locally Fermented Milk (Nono) Sold Under Market Conditions in Nasarawa State, Nigeria", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=IJSRON2013319, Volume 2 Issue 2, February 2013, 591 - 598
105 PDF Views | 98 PDF Downloads
Similar Articles with Keyword 'emerging'
Research Paper, Biology, India, Volume 3 Issue 11, November 2014
Pages: 1424 - 1430Antimicrobial Activity & Anti Oxidant Activity of Extracellularly Synthesized Silver Nanoparticles from Marine Derived Aeromonas salmonicida
Pradeepa V., Saranya N
Research Paper, Biology, India, Volume 6 Issue 1, January 2017
Pages: 1591 - 1593Fig Tree with Unrelated Fig Wasps
Aravind Krishnan K., Gayathri G. S., Sreedevi Amma
Research Paper, Biology, Nigeria, Volume 2 Issue 2, February 2013
Pages: 591 - 598Occurrence and Antibiogram of Escherichia Coli O157:H7 in Locally Fermented Milk (Nono) Sold Under Market Conditions in Nasarawa State, Nigeria
C. R. Reuben, E.C. Okolocha, M. Bello, H. Tanimu
Research Paper, Biology, Bahrain, Volume 4 Issue 3, March 2015
Pages: 182 - 189Biosynthesis of Different Sizes of Silver Nanoparticles by Bacteria Screened from Cultivated Soil
Salwa Al-Thawadi, Sakina Mustafa, Mohammed Bououdina
Review Papers, Biology, Morocco, Volume 9 Issue 4, April 2020
Pages: 453 - 457COVID-19 : A Global Emergency and a Common Destiny between the Nations
H Chemsi, S Ibnmoussa, Y Sekhsokh, N Khlil
Similar Articles with Keyword 'Antibiotics'
Review Papers, Biology, Azerbaijan, Volume 5 Issue 4, April 2016
Pages: 2276 - 2279Modern Conceptions about Antibiotic Levorin A and its Derivatives Action in Cell and Bilayer Lipid Membranes
Dr. Samedova Arifa Ali Hasan, Dr. Sultanova Gulnar Hadjibek
Research Paper, Biology, Congo, Volume 4 Issue 11, November 2015
Pages: 1350 - 1352Comparative Study to Amino Glycoside Antibiotics Resistance of Staphylococci Strains Isolated from Wounds at Brazzaville
Rachel Moyen, Etienne Nguimbi, Gabriel Ahombo, Nina Esther Ontsira, Claudette Gatsele Yala, Obengui, Simon Charles Kobawila, Delphin Louembe
Research Paper, Biology, Morocco, Volume 4 Issue 5, May 2015
Pages: 2418 - 2423Microscopic Observation of the Effect of ZnO Nanoparticles Synthesized from Different Precursors on Eukaryotic and Prokaryotic Cells
Najia Moukrad, Fouzia Rhazi Filali, Omar Zegaoui, Ikram Daou
Research Paper, Biology, Iraq, Volume 6 Issue 9, September 2017
Pages: 877 - 880Isolation and Identification of Micro- Flora Streptococci from Chicken Intestine and the Effects of Antibiotic and Heavy Metals on Growth
Eman A. Mukhaifi, Salwa A. Abdul Jaleel
Research Paper, Biology, India, Volume 4 Issue 4, April 2015
Pages: 1950 - 1954An Integrative Approach for In Silico Study on Treating Molluscum Contagiosum with Snail Slime
Deepak Tom Jose, Caroline Nirmala. R
Similar Articles with Keyword 'resistance'
Informative Article, Biology, Tanzania, Volume 3 Issue 7, July 2014
Pages: 1150 - 1154In vitro Antibacterial and Antifungal Activity of Tanzanian Bersama abyssinica
Never Zekeya, Francis Shahada, Musa Chacha
Research Paper, Biology, India, Volume 3 Issue 8, August 2014
Pages: 1972 - 1974Inhibition of Antibiofilm Mediated Virulence Factors Production in Urinary Pathogen Serratia marcescens by Vitex Trifolia
R. Nancy Immaculate Mary, N. Banu
Research Paper, Biology, Iraq, Volume 4 Issue 4, April 2015
Pages: 2652 - 2656ABO Blood Group System Polymorphism and its Relation to Diseases Affection in Diyala Province
S. H. Alsamarrae Anas A. Humadi
Review Papers, Biology, India, Volume 3 Issue 9, September 2014
Pages: 2238 - 2245Diabetes and Progression of Alzheimer?s: Linking the Two Epidemics
Dr. S. S. Bandyopadhyay, Hiral Mistry, Rudra Prasad Dutta
Research Paper, Biology, Congo, Volume 4 Issue 11, November 2015
Pages: 1350 - 1352Comparative Study to Amino Glycoside Antibiotics Resistance of Staphylococci Strains Isolated from Wounds at Brazzaville
Rachel Moyen, Etienne Nguimbi, Gabriel Ahombo, Nina Esther Ontsira, Claudette Gatsele Yala, Obengui, Simon Charles Kobawila, Delphin Louembe