International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064




Downloads: 98

Research Paper | Medical Microbiology | Nigeria | Volume 8 Issue 8, August 2019


Determination of Azole Resistant Gene (ERG11) in Candida Species Isolated from Non-Pregnant Women Attending Amassoma Community Hospitals, Bayelsa State, Nigeria

Oluwayemisi A. Olorode, Nkechi O. Ezenobi, Bena Stanley


Abstract: Candida species are dimorphic organism in which they can exist as moulds and yeast. They have the ability to cause infection in individuals with impaired immunity. This study was carried out between January and August, 2018 to determine the presence of azole resistant gene (ERG11) in Candida species isolated from non-pregnant women between ages 18 and 36 attending Amassoma Community hospitals, Bayelsa state, Nigeria. A total of 150 higher vagina swabs were collected from 150 subjects and immediately transported to the laboratory for culture using Sabouraud Dextrose Agar. Pure isolates were characterised and identified on the basis of Gram Staining, Germ tube test, molecular analysis for the presence of ITS (Internal Transcriber Spacer) gene and CHROMagar Candida. Antifungal susceptibility testing was conducted by standard procedures and molecular analysis using polymerase chain reaction to reveal the resistant gene ERG11. A total of 92 (77 %) of Candida species were isolated; Candida albicans 72 (78 %), Candida krusei 15 (16 %), Candida glabrata 5 (5 %). Most of the Candida species isolated were susceptible to Ketoconazole (92.8 %) followed by fluconazole (66.6 %) and the least itraconazole (27 %). Resistant strains among the Candida species expressed ERG11 genes. Observation showed that women of ages 18 to 27years had the highest Candida infection this is followed by ages of 28 to 33years and the least women with Candidiasis were of ages 34 to 36years. In conclusion, this study has revealed ketoconazole as the most potent antifungal that can be prescribed for clinical use. Also antifungal resistant gene was common among the Candida species isolated in the study. Further study should be carried out to combat this effect of resistance.


Keywords: Candida infections, Non- Pregnant women, Resistance genes, Antifungal Susceptibility Testing, Higher Vaginal Swab


Edition: Volume 8 Issue 8, August 2019,


Pages: 1702 - 1708


How to Cite this Article?

Oluwayemisi A. Olorode, Nkechi O. Ezenobi, Bena Stanley, "Determination of Azole Resistant Gene (ERG11) in Candida Species Isolated from Non-Pregnant Women Attending Amassoma Community Hospitals, Bayelsa State, Nigeria", International Journal of Science and Research (IJSR), https://www.ijsr.net/get_abstract.php?paper_id=ART2020517, Volume 8 Issue 8, August 2019, 1702 - 1708

How to Share this Article?

Enter Your Email Address


Similar Articles with Keyword 'Candida'

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Medical Microbiology, India, Volume 10 Issue 11, November 2021

Pages: 1224 - 1229

Microbiological Profile and Antimicrobial Susceptibility of Microorganisms Isolated From Catheter Associated Urinary Tract Infections in an Adult Intensive Care Unit of Tertiary Care Center in Jaipur

Natwar Kumar Swarnkar, Aruna Vyas, Rajni Sharma, Jairam Choudhary

Share this Article

Downloads: 40

Research Paper, Medical Microbiology, India, Volume 10 Issue 4, April 2021

Pages: 110 - 116

Microbiological Trends of Candidemia and Antifungal Susceptibility Pattern: Seven Years Experience at a Multi Super - Specialty Center in Northern India

Eshani Dewan, Vandana Verma

Share this Article

Similar Articles with Keyword 'infections'

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Medical Microbiology, India, Volume 10 Issue 11, November 2021

Pages: 1224 - 1229

Microbiological Profile and Antimicrobial Susceptibility of Microorganisms Isolated From Catheter Associated Urinary Tract Infections in an Adult Intensive Care Unit of Tertiary Care Center in Jaipur

Natwar Kumar Swarnkar, Aruna Vyas, Rajni Sharma, Jairam Choudhary

Share this Article

Downloads: 38

Research Paper, Medical Microbiology, India, Volume 10 Issue 2, February 2021

Pages: 976 - 978

Detection of Pyogenic Infections among Clinical Samples Received in Department of Microbiology in Integral Institute of Medical Sciences and Research, Lucknow, Uttar Pradesh: A Retrospective Analysis

Nikita Mishra, Shilpi Srivastava, Namita Srivastava

Share this Article

Similar Articles with Keyword 'Non'

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Medical Microbiology, India, Volume 10 Issue 11, November 2021

Pages: 1224 - 1229

Microbiological Profile and Antimicrobial Susceptibility of Microorganisms Isolated From Catheter Associated Urinary Tract Infections in an Adult Intensive Care Unit of Tertiary Care Center in Jaipur

Natwar Kumar Swarnkar, Aruna Vyas, Rajni Sharma, Jairam Choudhary

Share this Article

Downloads: 24

Research Paper, Medical Microbiology, India, Volume 10 Issue 2, February 2021

Pages: 1536 - 1539

Analysis of Biofilm Formation and its Correlation with Antibiotic Susceptibility Pattern of Uropathogens

Smita Pokharnikar, Hemangi Walke, Neeta Jangale

Share this Article

Similar Articles with Keyword 'Pregnant'

Downloads: 80

Research Paper, Medical Microbiology, India, Volume 9 Issue 10, October 2020

Pages: 1448 - 1450

Seropositivity of Torch Infection in Patients with Bad Obstetrics History

Aakansha Pandey Shukla, Adityam Shukla

Share this Article

Downloads: 105

Research Paper, Medical Microbiology, India, Volume 7 Issue 6, June 2018

Pages: 1553 - 1555

Spectrum of Intestinal Parasitic Infection in HIV Infected Pregnant Women

Dr. Geetumoni Sonowal, Dr. Naba Kumar Hazarika

Share this Article

Similar Articles with Keyword 'women'

Downloads: 80

Research Paper, Medical Microbiology, India, Volume 9 Issue 10, October 2020

Pages: 1448 - 1450

Seropositivity of Torch Infection in Patients with Bad Obstetrics History

Aakansha Pandey Shukla, Adityam Shukla

Share this Article

Downloads: 105

Research Paper, Medical Microbiology, India, Volume 7 Issue 6, June 2018

Pages: 1553 - 1555

Spectrum of Intestinal Parasitic Infection in HIV Infected Pregnant Women

Dr. Geetumoni Sonowal, Dr. Naba Kumar Hazarika

Share this Article

Similar Articles with Keyword 'Resistance'

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Medical Microbiology, India, Volume 10 Issue 11, November 2021

Pages: 1224 - 1229

Microbiological Profile and Antimicrobial Susceptibility of Microorganisms Isolated From Catheter Associated Urinary Tract Infections in an Adult Intensive Care Unit of Tertiary Care Center in Jaipur

Natwar Kumar Swarnkar, Aruna Vyas, Rajni Sharma, Jairam Choudhary

Share this Article

Downloads: 1

Research Paper, Medical Microbiology, India, Volume 10 Issue 8, August 2021

Pages: 863 - 869

Antimicrobial Susceptibility Pattern of Multi Drug Resistant (MDR) Pseudomonas aeruginosa in a Tertiary Care Hospital from Central India

Manish Patidar, Dr. Naveen Dhingra

Share this Article

Similar Articles with Keyword 'genes'

Downloads: 24

Research Paper, Medical Microbiology, India, Volume 10 Issue 2, February 2021

Pages: 1536 - 1539

Analysis of Biofilm Formation and its Correlation with Antibiotic Susceptibility Pattern of Uropathogens

Smita Pokharnikar, Hemangi Walke, Neeta Jangale

Share this Article

Downloads: 107

Case Studies, Medical Microbiology, India, Volume 8 Issue 3, March 2019

Pages: 1624 - 1625

Case Series of Aerococcus urinae: A Rare Entity, its Role in UTI and its Successful Management in Healthy as Well as Immunocompromised Patients

Dr. Anu Sharma, Dr. Sonal Agarwal, Dr. N. K. Shaikh

Share this Article

Similar Articles with Keyword 'Antifungal'

Downloads: 40

Research Paper, Medical Microbiology, India, Volume 10 Issue 4, April 2021

Pages: 110 - 116

Microbiological Trends of Candidemia and Antifungal Susceptibility Pattern: Seven Years Experience at a Multi Super - Specialty Center in Northern India

Eshani Dewan, Vandana Verma

Share this Article

Downloads: 106

Research Paper, Medical Microbiology, India, Volume 6 Issue 4, April 2017

Pages: 1608 - 1609

New Threat in Newborns: Multiresistant Non-Albicans Candidemia

Dr Baishali Chakraborty, Dr Dibyendu Banerjee, Dr Sharmistha Koley, Dr Banya Chakraborty

Share this Article

Similar Articles with Keyword 'Susceptibility'

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Medical Microbiology, India, Volume 10 Issue 11, November 2021

Pages: 1224 - 1229

Microbiological Profile and Antimicrobial Susceptibility of Microorganisms Isolated From Catheter Associated Urinary Tract Infections in an Adult Intensive Care Unit of Tertiary Care Center in Jaipur

Natwar Kumar Swarnkar, Aruna Vyas, Rajni Sharma, Jairam Choudhary

Share this Article

Downloads: 1

Research Paper, Medical Microbiology, India, Volume 10 Issue 8, August 2021

Pages: 863 - 869

Antimicrobial Susceptibility Pattern of Multi Drug Resistant (MDR) Pseudomonas aeruginosa in a Tertiary Care Hospital from Central India

Manish Patidar, Dr. Naveen Dhingra

Share this Article

Similar Articles with Keyword 'Testing'

Downloads: 1 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1

Research Paper, Medical Microbiology, India, Volume 10 Issue 11, November 2021

Pages: 1224 - 1229

Microbiological Profile and Antimicrobial Susceptibility of Microorganisms Isolated From Catheter Associated Urinary Tract Infections in an Adult Intensive Care Unit of Tertiary Care Center in Jaipur

Natwar Kumar Swarnkar, Aruna Vyas, Rajni Sharma, Jairam Choudhary

Share this Article

Downloads: 1

Research Paper, Medical Microbiology, India, Volume 10 Issue 8, August 2021

Pages: 1196 - 1200

Uropathogens and the Common Resistance Mechanisms using Vitek 2 System: A Retrospective Study in North Goa District Hospital

Gauri Dukle, Patricia Fernandes, Nividha Fernandes, Varsha Munj

Share this Article

Similar Articles with Keyword 'Higher'

Downloads: 6 | Weekly Hits: ⮙1 | Monthly Hits: ⮙2

Review Papers, Medical Microbiology, India, Volume 10 Issue 7, July 2021

Pages: 227 - 230

Novel Coronavirus (COVID-19) - A Review

Adil Raza

Share this Article

Downloads: 24

Research Paper, Medical Microbiology, India, Volume 10 Issue 2, February 2021

Pages: 1536 - 1539

Analysis of Biofilm Formation and its Correlation with Antibiotic Susceptibility Pattern of Uropathogens

Smita Pokharnikar, Hemangi Walke, Neeta Jangale

Share this Article
Top