International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Since Year 2012 | Open Access | Double Blind Reviewed

ISSN: 2319-7064




Downloads: 263

Comparative Studies | Biotechnology | India | Volume 9 Issue 1, January 2020


A Comparative Study on Screening Methods for the Detection of Protease Activity Containing Bacteria

Niranjana J | Bavithra P.S


Abstract: Investigations were carried out to detect the proteolytic activity of the bacteria inoculated on substrate agar plates using different screening methods. This article focuses on the three methods for screening protease activity. The first method involves the flooding of bromocresol green reagent (BCG) on casein milk agar plates. Second, a minimum of 0.0015 % of BCG reagent was incorporated with casein milk agar plates and third, gelatin clearing zone plates flooded with mercuric chloride solution. As an outcome, the plates screened with BCG reagent showed clear zone with green-blue background was effective in determining protease activity containing bacteria when comparing to the plates that were colorless.


Keywords: Protease screening, Bromocresol green reagent, Casein milk agar, Gelatin agar plates


Edition: Volume 9 Issue 1, January 2020,


Pages: 169 - 171


How to Cite this Article?

Niranjana J, Bavithra P.S, "A Comparative Study on Screening Methods for the Detection of Protease Activity Containing Bacteria", International Journal of Science and Research (IJSR), Volume 9 Issue 1, January 2020, pp. 169-171, https://www.ijsr.net/get_abstract.php?paper_id=ART20203705

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