International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Most Trusted Research Journal Since Year 2012

ISSN: 2319-7064

Dissertation Chapters | Medical Sciences | Indonesia | Volume 8 Issue 9, September 2019

Extract of Water Clover (Marsilea crenata C.Presl) Leaves Reduced Expression of MMP-1 in Skin of Ovariectomized Sprague Dawley Rat

Nenden Lilis Setiasih, Wimpie Pangkahila, Anak Agung Gde Putra Wiraguna, Ida Sri Iswari

Science has found a way to prevent skin aging in menopause woman. One of the therapies is estrogen (17-estradiol) replacement therapy. Skin aging in menopause woman is related with reduced ER- and ER- expression and negatively correlated with MMP-1 expression. Syntethic estrogen has several negative effects like breast and ovarian cancer. Phytoestrogen has gain attention due fewer side effects and cheaper. Water clover (Marsilea crenata C.Presl) is easily found in Indonesia which has phytoestrogen properties (isoflavon). Based on our review there was no study about effect of extract of water clover (Marsilea crenata C.Presl) leaves on MMP-1 expression in skin of ovariectomized Sprague Dawley rat. This research revealed the effect of extract of water clover (Marsilea crenata C.Presl) leaves on MMP-1 expression in skin of ovariectomized Sprague Dawley rat. Thirty-six female rats (Sprague Dawley) with 10-12 weeks old and 250-300 gr weight were randomly chosen and adapted for 7 days. Rats were ovariectomized and healed for 7 days then divided into 2 groups. The plasebo group (control) was rats without intervention and treated grup (treatment) was rats with daily oral 50 mg/kg weight extract of water clove for 28 days. After 48 hours from the last exposure, the rats were sacrificed and MMP1 expression in skin was determined by immunohistochemistry. Independent T-test analysis found significant difference in percentage of fibroblast that expressed MMP1 (p =0.000). The control group expressed MMP1 for 35.099 % and the treatment group expressed MMP1 for 17.463 %. There was decreasing of MMP1 expression for 17.636 % between control and treatment group. This study showed that extract of water clover (Marsilea crenata C.Presl) leaves reduced expression of MMP1 in fibroblast of skin ovariectomized Sprague Dawley rat significantly.

Keywords: water clover leaves, aging, MMP-1, menopause

Edition: Volume 8 Issue 9, September 2019

Pages: 327 - 329


How to Cite this Article?

Nenden Lilis Setiasih, Wimpie Pangkahila, Anak Agung Gde Putra Wiraguna, Ida Sri Iswari, "Extract of Water Clover (Marsilea crenata C.Presl) Leaves Reduced Expression of MMP-1 in Skin of Ovariectomized Sprague Dawley Rat", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=ART20201018, Volume 8 Issue 9, September 2019, 327 - 329

62 PDF Views | 48 PDF Downloads

Download Article PDF



Similar Articles with Keyword 'aging'

Review Papers, Medical Sciences, India, Volume 7 Issue 9, September 2018

Pages: 954 - 958

A Review of Rasakalpas in the Management of Prameha

Mangala Jadhav, Sameeksha Rahate, Anjali Menon

Share this article

Research Paper, Medical Sciences, Saudi Arabia, Volume 2 Issue 2, February 2013

Pages: 588 - 590

Ultrasound Imaging in the Diagnosis and Assessment of Testicular Disease

Abdullah Hamda, Alsafi Abdulla, Mohamed Yousef

Share this article

Research Paper, Medical Sciences, Bangladesh, Volume 8 Issue 9, September 2019

Pages: 863 - 866

Spinal Tuberculosis in Pediatric Patients

Dr. Jagodish Chandra Ghosh

Share this article

Case Studies, Medical Sciences, India, Volume 8 Issue 9, September 2019

Pages: 1252 - 1255

A Clinical Study on Optic Neuritis in a Patient of Paediatrics Age Group Attending O.P.D. in a Tertiary Care Hospital in Assam - A Rare Case Report

Dr. Moushumi Majumder, Dr. Bikram Dam

Share this article

Case Studies, Medical Sciences, India, Volume 8 Issue 9, September 2019

Pages: 1545 - 1552

A Clinical Study on Correlation of Albuminuria with Different Stages of Diabetic Retinopathy

Kotcharlakota Divya, Alekhya Gurram

Share this article

Similar Articles with Keyword 'MMP-1'

Research Paper, Medical Sciences, India, Volume 7 Issue 11, November 2018

Pages: 1459 - 1461

The State of the System of Matrix Metalloproteinases and of their Tissue Inhibitors in Patients with Metabolic Syndrome

Magapu Veera Venkata Akhil

Share this article

Dissertation Chapters, Medical Sciences, Indonesia, Volume 8 Issue 9, September 2019

Pages: 327 - 329

Extract of Water Clover (Marsilea crenata C.Presl) Leaves Reduced Expression of MMP-1 in Skin of Ovariectomized Sprague Dawley Rat

Nenden Lilis Setiasih, Wimpie Pangkahila, Anak Agung Gde Putra Wiraguna, Ida Sri Iswari

Share this article

Similar Articles with Keyword 'menopause'

Dissertation Chapters, Medical Sciences, India, Volume 8 Issue 10, October 2019

Pages: 650 - 651

Menopause & Homoeopathy

Dr. Sonal Sinha

Share this article

Review Papers, Medical Sciences, Indonesia, Volume 8 Issue 7, July 2019

Pages: 1420 - 1422

Estrogen Receptors Expression in Lung Cancer and Breast Cancer

Ni Luh Gede Yoni Komalasari

Share this article

Dissertation Chapters, Medical Sciences, Indonesia, Volume 8 Issue 9, September 2019

Pages: 327 - 329

Extract of Water Clover (Marsilea crenata C.Presl) Leaves Reduced Expression of MMP-1 in Skin of Ovariectomized Sprague Dawley Rat

Nenden Lilis Setiasih, Wimpie Pangkahila, Anak Agung Gde Putra Wiraguna, Ida Sri Iswari

Share this article

Top