Research Paper | Microbiology Science | India | Volume 8 Issue 7, July 2019
Clinical and Microbiological Study of Different Corneal Ulcers
Dr Alekhya Gurram, Dr Kotcharlakota Divya, Dr Madhan Mohan Reddy
AIMS AND OBJECTIVES: Primary objective: To correlate between the clinical diagnosis and microbiological results of bacterial and fungal keratitis. Secondary objective: To identify predisposing factors responsible for bacterial and fungal infections of cornea. MATERIALS AND METHODS: Prospective study done in santhiram medical college and general hospital, NANDYAL between OCTOBER 2018 to July 2019. SAMPLE SIZE: 100 Method of collection of data: • Patients with infective corneal ulcers attending OPD/cornea-clinic of our hospital. • Informed written consent will be taken. • A detailed history including demographics, ocular disease, past medical illness, history of trauma, treatment history and personal history will be taken. • Ophthalmological examination will include: • Best corrected visual acuity • Slit lamp bio-microscopic examination • Digital IOP • Flourescein staining • All infectious corneal ulcers were subjected to smears & cultures. CLINICAL DIAGNOSIS: Out of 100 cases 22 were clinically diagnosed as bacterial, while 78 were diagnosed to be of fungal aetiology. SMEAR RESULTS: * 14 out of 22 clinically diagnosed bacterial ulcers were smear positive on gram stain. * Out of the 78 clinically diagnosed fungal corneal ulcers, 71 showed fungal hyphae on 10 % KOH wet mount. CULTURE RESULTS: • In 16 cases (19.04 %), bacteria were identified from the corneal scrapings cultures. • Cultures were positive for fungi in 72 cases (67.85 %). • Both bacterial & fungal growth in 1 case (1.19 %).. while no growth was detected in 11cases (11.90 %) Correlation between clinical diagnosis & microbiological cultures: 16 out of the clinically diagnosed bacterial ulcers yeilded growth on bacterial culture media thus giving % sensitivity to clinical diagnosis. sensitivity of clinically diagnosing fungal corneal ulcer is 82.35 %, as out of 78 clinically diagnosed fungal ulcers, 64 showed growth on SDA.
Keywords: corneal ulcers, sabourauds dextrose agar, gram stain, lactophenol cotton blue mount
Edition: Volume 8 Issue 7, July 2019
Pages: 85 - 92
How to Cite this Article?
Dr Alekhya Gurram, Dr Kotcharlakota Divya, Dr Madhan Mohan Reddy, "Clinical and Microbiological Study of Different Corneal Ulcers", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=ART20199257, Volume 8 Issue 7, July 2019, 85 - 92
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