Research Paper | Medical Microbiology | Nigeria | Volume 8 Issue 3, March 2019
Evaluation of Direct Nitrate Reductase Assay for Drug Susceptibility Testing of Mycobacterium tuberculosis in Resource - Poor Settings
Olubunmi Alaka | Anuoluwapo Alaka | Olamide Agboola | Augusta Onuoha
Abstract: Direct Nitrate Reductase Assay (DNRA) was used for susceptibility testing of Mycobacterium tuberculosis to two front line anti-tuberculosis drugs (Rifampicin and Isoniacid) from 100 Acid fast bacilli smear-positive sputum samples. The DNRA results were compared with the gold standard conventional proportion method (PM). Ninety eight (98) sputum specimens result were comparable with the gold standard. DNRA results were obtained at day 10 for 72 specimens, results for 17 specimens were obtained at day 14, and the results for 9 specimens were obtained at day 18. Thus, 74 % of DNRA results were obtained in 10 days. The sensitivities and specificities of DNRA were 100 %, 97 % and 100 %, 99 % for Rifampicin and Isoniacid respectively. Positive predictive values were 100 % and 93 % for Rifampicin and INH respectively while negative predictive values were 97 % and 100 % for Rifampicin and Isoniacid respectively. NRA has been demonstrated as rapid, accurate, and cost-effective method for drug susceptibility testing of Mycobacterium tuberculosis. Therefore, NRA constitutes a useful tool for detection of tuberculosis drug resistance in low-resource countries.
Keywords: Mycobacterium tuberculosis, Drug testing, Nitrate Reductase Assay
Edition: Volume 8 Issue 3, March 2019,
Pages: 635 - 639
How to Cite this Article?
Olubunmi Alaka, Anuoluwapo Alaka, Olamide Agboola, Augusta Onuoha, "Evaluation of Direct Nitrate Reductase Assay for Drug Susceptibility Testing of Mycobacterium tuberculosis in Resource - Poor Settings", International Journal of Science and Research (IJSR), https://www.ijsr.net/get_abstract.php?paper_id=ART20195968, Volume 8 Issue 3, March 2019, 635 - 639, #ijsrnet
How to Share this Article?
Similar Articles with Keyword 'tuberculosis'
Finding Out of Occult TB Cases using Extra Pulmonary Sample Aspirates by CBNAAT (Cartridge Based Nucleic Acid Amplification Test) in A & N Islands
Dr. S. P. Burma | Dr. Sunita Sharma | Dr. V. Neelaveni
Study of Various Microorganisms Isolated from Chronic Supperative Otitis Media among Indian Population
Juhee Bhatia | Aashish Sharma