International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064


Downloads: 127

Research Paper | Microbiology | India | Volume 8 Issue 2, February 2019


Detection of Chitinase on Chitin Agar Plates

Kumkum Verma, Neelam Garg


Abstract: Chitin polymer is the second most abundant polysaccharide on earth after cellulose. The linear insoluble structure of chitin is difficult to be degraded in nature. Hydrolysis of chitin by chemicals results in the production of various non-specific products and has been proved unsafe for the environment. Chitinase is the enzyme which hydrolyses chitin polymer into its oligosaccharides and then to its monomeric form i. e. into N-Acetyl glucosamine. Chitinase have received attention due to its applications in the medicine, agriculture, waste management and industries. When chitinase producing bacteria are isolated from various sources, there is a need to detect their chitinase activity by some convenient and efficient methods so that large number of isolates can be screened. In the present study, chitinase production by different bacterial isolates was detected by flooding the inoculated chitin agar plates with four different stains.


Keywords: Congo red, cotton blue, Grams iodine, safranin, analysis


Edition: Volume 8 Issue 2, February 2019,


Pages: 1186 - 1189


How to Cite this Article?

Kumkum Verma, Neelam Garg, "Detection of Chitinase on Chitin Agar Plates", International Journal of Science and Research (IJSR), https://www.ijsr.net/get_abstract.php?paper_id=ART20195381, Volume 8 Issue 2, February 2019, 1186 - 1189

How to Share this Article?

Enter Your Email Address


Similar Articles with Keyword 'Congo red'

Downloads: 97

Research Paper, Microbiology, India, Volume 4 Issue 6, June 2015

Pages: 2470 - 2471

Isolation, Antibiogram and Pathogenicity of E.Coli Associated with Calf Diarrhoea

R.P. Diwakar, R.K.Diwakar

Share this Article

Downloads: 118

Research Paper, Microbiology, Morocco, Volume 5 Issue 10, October 2016

Pages: 1156 - 1159

Comparison of Three Methods for the Detection of Biofilm Formation by Clinical Isolates of Staphylococcus aureus Isolated in Casablanca

Achmit Mohamed, Ait Mhand Rajaa, Zerouali Khalid, Mellouki Fouad, Rhallabi Naima

Share this Article

Similar Articles with Keyword 'analysis'

Downloads: 2

Research Paper, Microbiology, India, Volume 10 Issue 7, July 2021

Pages: 1501 - 1503

Detection of Antimicrobial Susceptibility Pattern of Staphylococcus aureus among Clinical Samples Received in Department of Microbiology in Integral Institute of Medical Sciences and Research, Lucknow, Uttar Pradesh: A Retrospective Analysis

Namita Srivastava, Shilpi Srivastava, Nikita Mishra

Share this Article

Downloads: 5

Research Paper, Microbiology, Nigeria, Volume 10 Issue 4, April 2021

Pages: 861 - 872

Preliminary Investigation into the Ability of Tropical Rhizobia to Degrade Petroleum Products as Environmental Pollutants and Chemical Bases for Some Syhnthetic Pesticides

Olusoji A. A., Olusoji O. C., Aminu-Taiwo B.R

Share this Article

Similar Articles with Keyword 'Congo'

Downloads: 97

Research Paper, Microbiology, India, Volume 4 Issue 6, June 2015

Pages: 2470 - 2471

Isolation, Antibiogram and Pathogenicity of E.Coli Associated with Calf Diarrhoea

R.P. Diwakar, R.K.Diwakar

Share this Article

Downloads: 110

Research Paper, Microbiology, Congo, Volume 5 Issue 1, January 2016

Pages: 1907 - 1912

Prevalence of Serologic Markers and Risk Factors for Hepatitis B Virus among Pregnant Women in Brazzaville, Congo

Brunel Monic ANGOUNDA, Amlia BOKILO DZIA, Luc Magloire Anicet BOUMBA, Clotaire ITOUA, Gabriel AHOMBO, Donatien MOUKASSA, Jean-Rosaire IBARA, Moulay Mustapha ENNAJI

Share this Article

Similar Articles with Keyword 'red'

Downloads: 0

Research Paper, Microbiology, India, Volume 10 Issue 10, October 2021

Pages: 76 - 78

Regular Hospital Infection Control Team Rounds - A Prime Factor in Early Detection of NICU Outbreak

Walke Hemangi, Jangale Neeta

Share this Article

Downloads: 1

Student Project, Microbiology, India, Volume 10 Issue 6, June 2021

Pages: 1585 - 1588

Role of Antibiotics against Staphylococcus Aureus

Ruturaj Solanki, Nimisha Patel

Share this Article

Similar Articles with Keyword 'cotton'

Downloads: 109

Research Paper, Microbiology, India, Volume 3 Issue 9, September 2014

Pages: 1413 - 1418

Optimization of Process Parameters for Lovastatin Production from Red Gram Bran by Solid State Fermentation

Velusamy Kavitha, Balraj Janani, Jayaraman Angayarkanni

Share this Article

Downloads: 111

Research Paper, Microbiology, India, Volume 3 Issue 6, June 2014

Pages: 1759 - 1763

Optimization; Purification of Cellulase Produced from Bacillus subtilis subsp. inaquosorum under Solid State Fermentation and its Potential Applications in Denim Industry

Richa Gautam, Jitender Sharma

Share this Article

Similar Articles with Keyword 'blue'

Downloads: 116

Research Paper, Microbiology, Iraq, https://www.ijsr.net/issue.php?edition=Volume%203%20Issue%208,%20August%20201

Pages: 935 - 939

Using of astA and uidA Genes Characterization in Detection of Escherichia coli Prevalence from Human Gallstone

Abdalnabi J. Abid, Sama J. AL-zuwainy

Share this Article

Downloads: 125

Research Paper, Microbiology, India, Volume 3 Issue 9, September 2014

Pages: 455 - 460

Unique level of Multi-Drug Resistance (MDR) in Pseudomonas aeruginosa Strain DEB1

Debasis Mitra, Arup Kumar Mondal, Arijit Mukhopadhyay

Share this Article

Similar Articles with Keyword 'Grams'

Downloads: 44

Research Paper, Microbiology, Nigeria, Volume 6 Issue 12, December 2017

Pages: 1484 - 1492

Phytochemical Determination and In Vitro Antimicrobial Activity of Crude Ethanolic Extract of Stem Bark of Boswellia dalzielii

Baha'uddeen Salisu Dandashire; Abdulkadir Magaji Magashi; Bashir Abdulkadir

Share this Article

Downloads: 110

Research Paper, Microbiology, Congo, Volume 5 Issue 1, January 2016

Pages: 1907 - 1912

Prevalence of Serologic Markers and Risk Factors for Hepatitis B Virus among Pregnant Women in Brazzaville, Congo

Brunel Monic ANGOUNDA, Amlia BOKILO DZIA, Luc Magloire Anicet BOUMBA, Clotaire ITOUA, Gabriel AHOMBO, Donatien MOUKASSA, Jean-Rosaire IBARA, Moulay Mustapha ENNAJI

Share this Article
Top