International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Since Year 2012 | Open Access | Double Blind Reviewed

ISSN: 2319-7064



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Research Paper | Pharmaceutical Science | India | Volume 7 Issue 9, September 2018


Stress Degradation Studies of Hydrochlorothiazide and Development of Validated Method by UV Spectroscopy

Devi.V [2] | Sekar.V


Abstract: To develop a simple, precise, accurate, and stability indicating a UV-method for estimation of Hydrochlorothiazide. In bulk and formulated dosage form. The drug was under subjected to stress degradation at different conditions recommended by the International Conference on Harmonization (ICH). The samples are generated and used for the degradation studies. The max of the Hydrochlorothiazide was found to be 273 nm. The linearity of calibration curve (Absorbance Vs Concentration) in pure solution was checked over the concentration ranges of about 5-30g/ml for Hydrochlorothiazide respectively, with the correlation coefficient higher than 0.99. The regression equation of the curve was Y = 0.598x + 0.0042. % RSD was found to be within the limit as per ICH guidelines. The obtained percentage recovery of Hydrochlorothiazide was found to be within the limit 100 % SD. The proposed method can be successfully applied for the method development, validation and stress degradation studies of Hydrochlorothiazide. The percentage degradation limit should be 5-20 %. The drug Hydrochlorothiazide was found to be within the limit.


Keywords: UV Spectroscopy, Hydrochlorothiazide, Stress degradation, validation


Edition: Volume 7 Issue 9, September 2018,


Pages: 403 - 406


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How to Cite this Article?

Devi.V, Sekar.V, "Stress Degradation Studies of Hydrochlorothiazide and Development of Validated Method by UV Spectroscopy", International Journal of Science and Research (IJSR), Volume 7 Issue 9, September 2018, pp. 403-406, https://www.ijsr.net/get_abstract.php?paper_id=ART20191170



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