A Comparative Study of Fluorescence Microscopy with Ziehl Neelsen Staining for Detection of Acid Fast Bacilli in Lymph Node Aspirates
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064



Downloads: 155 | Weekly Hits: ⮙4 | Monthly Hits: ⮙13

Comparative Studies | Pathology | India | Volume 7 Issue 6, June 2018

A Comparative Study of Fluorescence Microscopy with Ziehl Neelsen Staining for Detection of Acid Fast Bacilli in Lymph Node Aspirates

Supreet Kaur Kalra, Surinder Paul, S.P. Singh

Objective To study the cytomorphological features in fine needle aspiration smears from patients suspected of having tuberculous lymphadenitis using May Grunwald Giemsa (MGG) and Haematoxylin and Eosin stains and compare the efficacy of Ziehl Neelsen staining (ZN) and fluorescent staining for the detection of acid fast bacilli on lymph node aspirates. Materials and methods The present study was conducted in 100 patients presenting with peripheral lymphadenopathy suspicious of tuberculous lymphadenitis. Fine needle aspirations were performed and smears from the aspirates were processed for routine cytology, the conventional ZN method, and the fluorescent method. Results The smear positivity with ZN stain was seen in 37 % of cases while the positivity increased to 73 % with fluorescent method. Conclusion Fluorescent microscopy has the advantage of speed and ease of screening, and reduces observer fatigue. The fluorescent method was found to be more advantageous than routine cytology and conventional ZN method, particularly in paucibacillary cases.

Keywords: Ziehl Neelsen, Auramine-O, fluorescent microscopy, acid fast bacilli

Edition: Volume 7 Issue 6, June 2018

Pages: 789 - 795

Share this Article

How to Cite this Article?

Supreet Kaur Kalra, Surinder Paul, S.P. Singh, "A Comparative Study of Fluorescence Microscopy with Ziehl Neelsen Staining for Detection of Acid Fast Bacilli in Lymph Node Aspirates", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=ART20183250, Volume 7 Issue 6, June 2018, 789 - 795

Enter Your Registered Email Address





Similar Articles with Keyword 'Ziehl Neelsen'

Downloads: 124 | Weekly Hits: ⮙4

Research Paper, Pathology, India, Volume 4 Issue 9, September 2015

Pages: 222 - 225

Efficacy of Fluorescent Method over Conventional ZN Method in Detection of Acid Fast Bacilli among Various Cytomorphological Patterns of Tubercular Lymphadenitis

Dr. Chakkirala Nalini Mohan, Dr. Vamseedhar Annam, Dr. Nitin Gangane

Share this Article

Downloads: 155 | Weekly Hits: ⮙4 | Monthly Hits: ⮙13

Comparative Studies, Pathology, India, Volume 7 Issue 6, June 2018

Pages: 789 - 795

A Comparative Study of Fluorescence Microscopy with Ziehl Neelsen Staining for Detection of Acid Fast Bacilli in Lymph Node Aspirates

Supreet Kaur Kalra, Surinder Paul, S.P. Singh

Share this Article

Similar Articles with Keyword 'acid fast bacilli'

Downloads: 115 | Weekly Hits: ⮙2

Research Paper, Pathology, India, Volume 4 Issue 5, May 2015

Pages: 1211 - 1213

FNAC Diagnosis of Lymph Nodes: An Institutional Experience

Mangala Goneppanavar, Rajendra Singh Dhaka

Share this Article

Downloads: 124 | Weekly Hits: ⮙4

Research Paper, Pathology, India, Volume 4 Issue 9, September 2015

Pages: 222 - 225

Efficacy of Fluorescent Method over Conventional ZN Method in Detection of Acid Fast Bacilli among Various Cytomorphological Patterns of Tubercular Lymphadenitis

Dr. Chakkirala Nalini Mohan, Dr. Vamseedhar Annam, Dr. Nitin Gangane

Share this Article

Downloads: 155 | Weekly Hits: ⮙4 | Monthly Hits: ⮙13

Comparative Studies, Pathology, India, Volume 7 Issue 6, June 2018

Pages: 789 - 795

A Comparative Study of Fluorescence Microscopy with Ziehl Neelsen Staining for Detection of Acid Fast Bacilli in Lymph Node Aspirates

Supreet Kaur Kalra, Surinder Paul, S.P. Singh

Share this Article



Top