International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Since Year 2012 | Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064




Downloads: 134

M.Tech / M.E / PhD Thesis | Microbiology | Nigeria | Volume 7 Issue 5, May 2018


Molecular Detection of Methicillin Resistant Staphylococcus Aureus Isolated From Hospital Patients and Food Handlers in FCT, North Central, Nigeria

Anowai C. O. | Agarry O. O. | Akin-Osanaiye B. C.


Abstract: Antimicrobial susceptibility profile and molecular detection of methicillin resistant genes isolated from hospital patients and food handlers in Abuja, Nigeria was evaluated. Specimen collection, identification, isolation and characterization were done following standard microbiological procedures. Polymerase chain reaction (PCR) was employed for the detection of the mecA gene associated with methicillin resistance and other virulent marker genes associated with pathogenicity of S. aureus such as Panton-Valentine Leukocidin (PVL), BlaZ and Nuc genes. The result showed a 27.34 % prevalence rate of S. aureus isolates in Abuja metropolis, 38.02 % prevalence rate was observed in clinical samples while food handlers had 16.67 %. Isolates from hospital patients were more resistant to the antibiotics tested than those from the food handlers. Vancomycin was the most effective antibiotic for MRSA isolates from both clinical patients and food handlers while linezolid could be used as an alternative. This study also observed that all the MRSA isolates were resistant to more than three antibiotics (multidrug resistant). High percentage of the isolates (83.33 %) that had cefoxitin MIC of 6g/ml encoded mecA gene. Nuc gene was observed in 45.83 % of the isolates, 4.17 % encoded PVL gene while none of the isolates harbored the blaZ gene. The finds from this study showed a high prevalence of methicillin-resistance S. aureus from clinical patients and food handlers sampled and these sources could serve as source of MRSA dissemination in the community.


Keywords: Staphylococcus aureus, antibiotics resistance, mecA gene, hospital patients, food handlers, Abuja


Edition: Volume 7 Issue 5, May 2018,


Pages: 1401 - 1408

Molecular Detection of Methicillin Resistant Staphylococcus Aureus Isolated From Hospital Patients and Food Handlers in FCT, North Central, Nigeria


How to Cite this Article?

Anowai C. O., Agarry O. O., Akin-Osanaiye B. C., "Molecular Detection of Methicillin Resistant Staphylococcus Aureus Isolated From Hospital Patients and Food Handlers in FCT, North Central, Nigeria", International Journal of Science and Research (IJSR), https://www.ijsr.net/get_abstract.php?paper_id=ART20182795, Volume 7 Issue 5, May 2018, 1401 - 1408, #ijsrnet

How to Share this Article?

Enter Your Email Address




Similar Articles with Keyword 'Staphylococcus aureus'

Downloads: 139

Research Paper, Microbiology, Nigeria, Volume 9 Issue 6, June 2020

Pages: 742 - 745

Isolation of Common Nosocomialpathogens from Gate Pass Used in Aminu Kano Teaching Hospital and Bayero University Kano, Nigeria

Umar Adamu Hamza [2] | Binta Muhammad Aminu | Muzammil Bashir Mustapha

Share this Article

Downloads: 0

Masters Thesis, Microbiology, Nigeria, Volume 11 Issue 6, June 2022

Pages: 267 - 273

Determination of Antibacterial Activities of Spirulina Plantensis in Owo Local Government against Selected Pathogens

Sofowora Iyinoluwa E. | Daniels Adetoyosi O.

Share this Article


Top