International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Call for Papers | Open Access | Double Blind Reviewed

ISSN: 2319-7064


Downloads: 139

Research Paper | Medicine Science | Saudi Arabia | Volume 7 Issue 2, February 2018


Hypertonic Saline Infusion Minimizes Bacterial Translocation and Histological Degenerative Changes in Liver of Shocked Rats

Abeer Nasser M Alghalbi


Abstract: Background The hypertonic saline (HTS) solutions have recently gained widespread acceptance in control of shock. Aim of the work The aim of this work is to study the use of hypertonic saline with the resuscitation fluids in rats with induced hemorrhagic shock to evaluate the impact of this solution on the extent of early bacterial translocation, blood pressure, and histopathological liver changes. Materials and methods sixty phenobarbital anesthetized rats were involved in this study where they were allowed to bleed. Arterial pressure was maintained below 50 mm Hg for 30 minutes. They were randomized into 2 groups, each of 30 rats. Resuscitation in group I was done by using Lactated Ringer & #039, s (LR) solution (60 ml/Kg) and in group II HTS 7.5 % in a dose of 4 ml /kg body weight was added to Lactated Ringer & #039, s (LR) solution (60 ml/Kg). Regular monitoring of blood pressure was done and blood samples were withdrawn 1, 30, and 90 minutes and 6 hours after commencement of the resuscitation and sent for blood culture for both aerobic and anaerobic growths. After 24 hours the liver was resected and sent for histological examination. Results The mean arterial blood pressure in group I before resuscitation was found to be about 4811 mm Hg and it was raised to a mean of 908 mm Hg after 2 hours of resuscitation with LR alone. In group II, the mean arterial blood pressure was found to be 495 mm Hg and it was efficiently controlled by HTS with LR and 2 hours after commencement of resuscitation it was elevated to 11410 mm Hg. The blood cultures were positive in 3.3 % of the rats of group II and in 40 % of rats of group I. Escherichia coli were the most commonly isolated organism. Histopathological findings showed inflammatory process and early severe hepatocyte degeneration of the liver of group I rats and these changes were absent or minimal in group II. Conclusion hypertonic saline was found to be effective for decreasing the rate of early bacterial translocation to blood and also for more efficient restoring of the mean arterial pressure in rats with induced hemorrhagic shock, in addition, HTS infusion minimizes the inflammatory and degenerative process in liver.


Keywords: Hypertonic saline, hepatocytes, bacterial translocation, rats


Edition: Volume 7 Issue 2, February 2018,


Pages: 377 - 381


How to Download this Article?

You Need to Register Your Email Address Before You Can Download the Article PDF


How to Cite this Article?

Abeer Nasser M Alghalbi, "Hypertonic Saline Infusion Minimizes Bacterial Translocation and Histological Degenerative Changes in Liver of Shocked Rats", International Journal of Science and Research (IJSR), Volume 7 Issue 2, February 2018, pp. 377-381, https://www.ijsr.net/get_abstract.php?paper_id=ART20179943

Similar Articles with Keyword 'Hypertonic saline'

Downloads: 113

Research Paper, Medicine Science, India, Volume 3 Issue 12, December 2014

Pages: 1115 - 1119

A Pilot Study to Compare Effects of Mannitol with Hypertonic Saline in Combination with HES on Blood Coagulation and Platelet Function during Elective Craniotomy

Vidhu Bhatnagar [3]

Share this Article

Downloads: 114

Case Studies, Medicine Science, India, Volume 3 Issue 5, May 2014

Pages: 1492 - 1495

Cetrimide Induced Metabolic Acidosis: A Rare Intraoperative Complication in a Case of Hydatid Disease of Liver

Ketul S. Puj | Vineet F. Chauhan

Share this Article
Top