Development and Validation of Liquid Chromatography-Tandem Mass Spectrometric Method for Simultaneous Quantitation of Mangiferin and Isoquercetin from Dried Leaf Powder Extract of Bombax ceiba Linn
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064



Downloads: 124 | Monthly Hits: ⮙8

Research Paper | Chemistry | India | Volume 7 Issue 1, January 2018

Development and Validation of Liquid Chromatography-Tandem Mass Spectrometric Method for Simultaneous Quantitation of Mangiferin and Isoquercetin from Dried Leaf Powder Extract of Bombax ceiba Linn

Deepti Bhandarkar, Vidya Dighe, Ajit Datar

A rapid, sensitive, and accurate liquid chromatography-tandem mass spectrometric (LC-MS/MS) method is developed for simultaneous quantitation of mangiferin and isoquercetin from dried leaf powder extract of Bombax ceiba Linn. The chromatographic separation was carried out using a Shim-pack XR ODS (75 mm L x 3 mm ID, 2.2 m) column. The mobile phase used was 0.1 % formic acid in water and acetonitrile at a flow rate of 0.5 mL/min in a gradient elution mode. Nexera UHPLC system coupled with LCMS-8040 triple quadrupole mass spectrometer with Electro Spray Ionization (ESI) source was used as a detector in Multiple Reaction Monitoring (MRM) mode. The method was found to be simple, precise, accurate, fast, specific, and sensitive that can be used for routine quality control of dried leaf powder extract of Bombax ceiba Linn.

Keywords: LC-MS/MS, mangiferin, isoquercetin, MRM, Bombax ceiba Linn

Edition: Volume 7 Issue 1, January 2018

Pages: 1562 - 1566

Share this Article

How to Cite this Article?

Deepti Bhandarkar, Vidya Dighe, Ajit Datar, "Development and Validation of Liquid Chromatography-Tandem Mass Spectrometric Method for Simultaneous Quantitation of Mangiferin and Isoquercetin from Dried Leaf Powder Extract of Bombax ceiba Linn", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=ART20179733, Volume 7 Issue 1, January 2018, 1562 - 1566

Enter Your Registered Email Address





Similar Articles with Keyword 'LC-MS/MS'

Downloads: 124 | Monthly Hits: ⮙8

Research Paper, Chemistry, India, Volume 7 Issue 1, January 2018

Pages: 1562 - 1566

Development and Validation of Liquid Chromatography-Tandem Mass Spectrometric Method for Simultaneous Quantitation of Mangiferin and Isoquercetin from Dried Leaf Powder Extract of Bombax ceiba Linn

Deepti Bhandarkar, Vidya Dighe, Ajit Datar

Share this Article

Downloads: 140 | Weekly Hits: ⮙3

Research Paper, Chemistry, India, Volume 6 Issue 7, July 2017

Pages: 655 - 658

Development of an LC-MS/MS Method for Trace Level Quantification of 4-(bromomethyl) Benzoic Acid, a Potential Genotoxic Impurity in Imatinib Mesylate Drug Substance

Nelaturi Subbaiah, G. Jegan, P. Suresh Babu, J Jayachandran, Nitesh Kanyawar, Manish Gangrade

Share this Article

Downloads: 146 | Weekly Hits: ⮙2 | Monthly Hits: ⮙12

Research Paper, Chemistry, India, Volume 6 Issue 3, March 2017

Pages: 162 - 166

A Selective and Sensitive Method Development and Validation by LC?MS/MS Approach for Trace Level Quantification of Potential Genotoxic Impurity IMT-01 in Imatinib Mesylate Drug Substance

Nelaturi Subbaiah, G. V. Subba Reddy

Share this Article

Downloads: 169 | Weekly Hits: ⮙2 | Monthly Hits: ⮙14

Review Papers, Chemistry, India, Volume 7 Issue 3, March 2018

Pages: 78 - 81

Review on Analytical Techniques used for the Estimation and Persistence of Ready-Mix Formulation Residues in Various Crops and Soil

Anita, Bharti Sharma

Share this Article

Similar Articles with Keyword 'MRM'

Downloads: 124 | Monthly Hits: ⮙8

Research Paper, Chemistry, India, Volume 7 Issue 1, January 2018

Pages: 1562 - 1566

Development and Validation of Liquid Chromatography-Tandem Mass Spectrometric Method for Simultaneous Quantitation of Mangiferin and Isoquercetin from Dried Leaf Powder Extract of Bombax ceiba Linn

Deepti Bhandarkar, Vidya Dighe, Ajit Datar

Share this Article

Downloads: 140 | Weekly Hits: ⮙3

Research Paper, Chemistry, India, Volume 6 Issue 7, July 2017

Pages: 655 - 658

Development of an LC-MS/MS Method for Trace Level Quantification of 4-(bromomethyl) Benzoic Acid, a Potential Genotoxic Impurity in Imatinib Mesylate Drug Substance

Nelaturi Subbaiah, G. Jegan, P. Suresh Babu, J Jayachandran, Nitesh Kanyawar, Manish Gangrade

Share this Article

Downloads: 146 | Weekly Hits: ⮙2 | Monthly Hits: ⮙12

Research Paper, Chemistry, India, Volume 6 Issue 3, March 2017

Pages: 162 - 166

A Selective and Sensitive Method Development and Validation by LC?MS/MS Approach for Trace Level Quantification of Potential Genotoxic Impurity IMT-01 in Imatinib Mesylate Drug Substance

Nelaturi Subbaiah, G. V. Subba Reddy

Share this Article



Top