Abstract: Molecular Study of Cellulase Gene Transcription Regulatory Elements via EMS Mutagenesis in a Novelthermophilic Cellulytic Bacillus sp.
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064

Downloads: 102

Research Paper | Biotechnology | Iraq | Volume 6 Issue 12, December 2017

Molecular Study of Cellulase Gene Transcription Regulatory Elements via EMS Mutagenesis in a Novelthermophilic Cellulytic Bacillus sp.

Ebtesam Basheer, Ehab Salman

Cellulose is the most common biomass on the globe and is available as huge amounts of agricultural and forestry natural residues. Microbial cellulases are the major enzymes widely used in degrading cellulose. Naturally cellulytic microorganisms need to be induced to increase their cellulytic activity. In this study, EMS chemical mutagen was used to increase cellulytic activity in the novel thermophilic Bacillus sp. isolate isolated from Iraqi soil. Sequencing results of cellulase gene transcription regulatory elements post-EMS mutagenesis showed a change in the nucleotides sequence. This change was accompanied by a significant increase in cellulase quantitative activity and total cellulase activity.

Keywords: Cellulase gene, transcription regulatory element, thermophilic cellulytic Bacillus

Edition: Volume 6 Issue 12, December 2017

Pages: 1664 - 1670

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How to Cite this Article?

Ebtesam Basheer, Ehab Salman, "Molecular Study of Cellulase Gene Transcription Regulatory Elements via EMS Mutagenesis in a Novelthermophilic Cellulytic Bacillus sp.", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=ART20178019, Volume 6 Issue 12, December 2017, 1664 - 1670

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