Research Paper | Biology | Iraq | Volume 6 Issue 10, October 2017
Distribution of Pathogenic Escherichia coli among Children with Severe Diarrhea in Al-Najaf Al- Ashraf City, Iraq
Massuma Sadiq Al Mussawi, Ahlam Kadhum Al-Yasseen
Diarrhea is considered an abnormal fecal discharge characterized by a frequent andor fluid stool. E.Coli is the most important bacterial cause of diarrhea in children, its remain harmlessly confined to the intestinal lumen. However, in the debilitated or immunosuppressed host, or when gastrointestinal barriers are violated, even the normal nonpathogenic strains of E.coli can cause infection. This study aimed to investigate the distribution of pathogenic E.coli among children suffering from severe diarrhea by detection of some their virulence genes. A total of 204 stool samples were collected from children with severe diarrhea under 5 years whom admitted to AL-Zahraa and Al-Hakeem hospital during a period from 3/9/2016 to 30/11/2016. All samples were cultured on Red and Green chrom agar for isolation of pathogenic E. coli. PCR technique has been carried out for detection ofeaeA, stx1, stx2 vtx1, vtx2 and Pcvd432 in 50 isolates that selective randomly. One hundred and seventy six isolates were belonging to pathogenic E. coli. The results of the amplification of 50 DNA samples showed that 8 (16 %) isolates were possessed eaeA gene while 1 (2 %) and 6 (12 %) isolates were carried stx1 and stx2 gene respectively whereas 7 (14 %) and 1 (2 %) isolates were possess pcvd432 and vtx2 respectively. diarrhea and bloody diarrhea were widely distributed in children in Al-Najaf Al-Ashraf city and mainly associated with presence of Atypical E. coli, Shiga-Like Toxin E.coli and Vero-Like Toxin E. coli.
Keywords: PECE, STEC, EHEC, Diarrhea, PCR
Edition: Volume 6 Issue 10, October 2017
Pages: 2075 - 2079
How to Cite this Article?
Massuma Sadiq Al Mussawi, Ahlam Kadhum Al-Yasseen, "Distribution of Pathogenic Escherichia coli among Children with Severe Diarrhea in Al-Najaf Al- Ashraf City, Iraq", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=ART20177732, Volume 6 Issue 10, October 2017, 2075 - 2079
128 PDF Views | 101 PDF Downloads
Similar Articles with Keyword 'Diarrhea'
Research Paper, Biology, Kenya, Volume 4 Issue 8, August 2015
Pages: 1161 - 1164Distribution of Colonization Factors and Toxins among Diarrheal Children below 5Years: A Case Study of Moi Teaching and Referral Hospital, Kenya
Faith Jeruto, Dr. Lizzy Mwamburi, Dr. James Wanga
Research Paper, Biology, Iraq, Volume 6 Issue 10, October 2017
Pages: 2075 - 2079Distribution of Pathogenic Escherichia coli among Children with Severe Diarrhea in Al-Najaf Al- Ashraf City, Iraq
Massuma Sadiq Al Mussawi, Ahlam Kadhum Al-Yasseen
Research Paper, Biology, India, Volume 5 Issue 8, August 2016
Pages: 1193 - 1194Bacteriological Study of Drinking Water in South Indore City (M. P.)
Ram Prajapati, Reena Rokde
Research Paper, Biology, Nigeria, Volume 5 Issue 8, August 2016
Pages: 1950 - 1955Clinical Safety Profiles of Artesunate and Amodiaquine in Combination with Sulphadoxine-Pyrimethamine for the Treatment of Uncomplicated Plasmodium Falciparum Malaria in Children
M. Kokori, M. M. Suleiman, L. S. Kela
Research Paper, Biology, Iraq, Volume 4 Issue 11, November 2015
Pages: 978 - 982Prevalence of Rotavirus Infection among Diarrheal Children in Baghdad City
Rawaa Abdulkhaleq Hussein, Areej Abd Al-Abbas, Arwa Mujahid Abdullah, Nada Taha Al-Bashier
Similar Articles with Keyword 'PCR'
Research Paper, Biology, Kenya, Volume 4 Issue 8, August 2015
Pages: 1161 - 1164Distribution of Colonization Factors and Toxins among Diarrheal Children below 5Years: A Case Study of Moi Teaching and Referral Hospital, Kenya
Faith Jeruto, Dr. Lizzy Mwamburi, Dr. James Wanga
Research Paper, Biology, Iraq, Volume 6 Issue 10, October 2017
Pages: 2075 - 2079Distribution of Pathogenic Escherichia coli among Children with Severe Diarrhea in Al-Najaf Al- Ashraf City, Iraq
Massuma Sadiq Al Mussawi, Ahlam Kadhum Al-Yasseen
Research Paper, Biology, India, Volume 4 Issue 9, September 2015
Pages: 1311 - 1314Genetic Diversity of Himalayan Region Chicken through SSR Molecular Markers
Aman Singh Bhalla, Mritunjay Kumar Singh, Ahmed Kareem Alatafi, Roma Lal, Aiyappa Parvangada
Research Paper, Biology, India, Volume 4 Issue 12, December 2015
Pages: 2181 - 2187Isolation and Molecular Characterization of Entomopathogenic Nematode (Steinernema Siamkayai)
Swati, Shailendra Singh Gaurav
Research Paper, Biology, Macedonia, Volume 3 Issue 12, December 2014
Pages: 1474 - 1476Monitoring of Immune Parameters in Patients with Bronchial Asthma in Tetovo Area
Enver Zenku, Sheval Memishi, Ismije Saiti