Research Paper | Biology Science | Iraq | Volume 6 Issue 10, October 2017
Distribution of Pathogenic Escherichia coli among Children with Severe Diarrhea in Al-Najaf Al- Ashraf City, Iraq
Massuma Sadiq Al Mussawi, Ahlam Kadhum Al-Yasseen
Diarrhea is considered an abnormal fecal discharge characterized by a frequent andor fluid stool. E.Coli is the most important bacterial cause of diarrhea in children, its remain harmlessly confined to the intestinal lumen. However, in the debilitated or immunosuppressed host, or when gastrointestinal barriers are violated, even the normal nonpathogenic strains of E.coli can cause infection. This study aimed to investigate the distribution of pathogenic E.coli among children suffering from severe diarrhea by detection of some their virulence genes. A total of 204 stool samples were collected from children with severe diarrhea under 5 years whom admitted to AL-Zahraa and Al-Hakeem hospital during a period from 3/9/2016 to 30/11/2016. All samples were cultured on Red and Green chrom agar for isolation of pathogenic E. coli. PCR technique has been carried out for detection ofeaeA, stx1, stx2 vtx1, vtx2 and Pcvd432 in 50 isolates that selective randomly. One hundred and seventy six isolates were belonging to pathogenic E. coli. The results of the amplification of 50 DNA samples showed that 8 (16 %) isolates were possessed eaeA gene while 1 (2 %) and 6 (12 %) isolates were carried stx1 and stx2 gene respectively whereas 7 (14 %) and 1 (2 %) isolates were possess pcvd432 and vtx2 respectively. diarrhea and bloody diarrhea were widely distributed in children in Al-Najaf Al-Ashraf city and mainly associated with presence of Atypical E. coli, Shiga-Like Toxin E.coli and Vero-Like Toxin E. coli.
Keywords: PECE, STEC, EHEC, Diarrhea, PCR
Edition: Volume 6 Issue 10, October 2017
Pages: 2075 - 2079
How to Cite this Article?
Massuma Sadiq Al Mussawi, Ahlam Kadhum Al-Yasseen, "Distribution of Pathogenic Escherichia coli among Children with Severe Diarrhea in Al-Najaf Al- Ashraf City, Iraq", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=ART20177732, Volume 6 Issue 10, October 2017, 2075 - 2079
14 PDF Views | 18 PDF Downloads
Similar Articles with Keyword 'Diarrhea'
Clinical Safety Profiles of Artesunate and Amodiaquine in Combination with Sulphadoxine-Pyrimethamine for the Treatment of Uncomplicated Plasmodium Falciparum Malaria in Children
M. Kokori, M. M. Suleiman, L. S. Kela
Prevalence of Rotavirus Infection among Diarrheal Children in Baghdad City
Rawaa Abdulkhaleq Hussein, Areej Abd Al-Abbas, Arwa Mujahid Abdullah, Nada Taha Al-Bashier
Development of Tomato Sauce Fortified with Bael and its Sensory and Chemical Evaluation
Pingale Sangeeta Avinash, Dighe Nilesh Madhav
Bacteriological Study of Drinking Water in South Indore City (M. P.)
Ram Prajapati, Reena Rokde
Diagnostic Procedures, Epidemiology and Genetic Diversity of Cryptosporidium Species in Bungoma County, Kenya
Wasike Wekesa Eric, Kutima H L, Muya M S, Wamachi A
Similar Articles with Keyword 'PCR'
Genetic Diversity of Himalayan Region Chicken through SSR Molecular Markers
Aman Singh Bhalla, Mritunjay Kumar Singh, Ahmed Kareem Alatafi, Roma Lal, Aiyappa Parvangada
Clinical Development of Biomarker To Detect Oral Carcinoma In Relation To Genetic Polymorphism At MMP-9
Pradip Kumar Majumdar, Pritam Sukul, Sandhini Saha, Rohit Verma, Supriya Sen Gupta, Supriya Nath, Bijan Basak, Krishnendu Royh
Monitoring of Immune Parameters in Patients with Bronchial Asthma in Tetovo Area
Enver Zenku, Sheval Memishi, Ismije Saiti
Identification of Tomato Leaf Curl Virus (ToLCV) Strain Causing ToLCV in Tomato and Chilli
A. K. Chavan, R. S. Choudhary, R. B. Narwade, R. H. Autade
DNA Sequencing of Toxoplasma gondii in Slaughtered Animals (Cattle, Sheep and Goat) in Wasit Province - Iraq
Altamemy, Abdulkareem Aakool, Suadadbresam Khiry