International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Most Trusted Research Journal Since Year 2012

ISSN: 2319-7064



Research Paper | Chemistry Science | Iraq | Volume 6 Issue 2, February 2017

Estimation of Cortisone Acetate in Pharmaceutical Anti-inflammatory Drugs by HPLC-UV Technique

H. N. K. AL-Salman, Shaker.A.N.AL-Jadaan

The HPLC-UV System was used to assay of Cortisone acetate in Oral Tablets So, compare the bioavailability of two types of Cortisone acetate, commercial formulations and Cortisone acetate analar grade as a test formulation. Cortisone acetate concentrations were analyzed by HPLC-UV System at (=254 nm). The separation was achieved by using the Ion Pac Ercus C18 RP-Column, 5m, 4.6250 mm. The mobile phase consisted of water /acetonitrile (5545). The study of bioequivalence between the two Cortisone acetate formulations were assessed by calculating peaks height. The standard Cortisone acetate eluted at a flow rate of 1.0 ml/min. The method was found to belinear in the range (0.5 to 2.5) g/ml (n = 5) with R2 0.9991, also, the recoveries were range within 99-100 %. The detection limit of quantification (LLOQ) was 0.07909g/ml and lower limit of detection (LLOD) 0.02610 g/ml. showing average intra assay and inter-assay coefficients of RSD % about 0.522 %. The results of recoveries, RSD, and statistical parameters obtained in this study. The accurate, precise and sensitive refers to validation method for determination of Cortisone acetate in anti-inflammatory Pharmaceutical drugs.

Keywords: Cortisone acetate, Oral Tablets, formulation, and HPLC- UV System

Edition: Volume 6 Issue 2, February 2017

Pages: 1178 - 1183

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How to Cite this Article?

H. N. K. AL-Salman, Shaker.A.N.AL-Jadaan, "Estimation of Cortisone Acetate in Pharmaceutical Anti-inflammatory Drugs by HPLC-UV Technique", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=ART2017677, Volume 6 Issue 2, February 2017, 1178 - 1183

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Research Paper, Chemistry Science, Iraq, Volume 6 Issue 2, February 2017

Pages: 1178 - 1183

Estimation of Cortisone Acetate in Pharmaceutical Anti-inflammatory Drugs by HPLC-UV Technique

H. N. K. AL-Salman, Shaker.A.N.AL-Jadaan

Share this Article



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