Downloads: 126 | Views: 133
Research Paper | Biochemistry Science | Egypt | Volume 6 Issue 6, June 2017
Generation of Cholinergic Neurons from Human Olfactory Bulb Neural Stem Cells Using BMP9 Treatment
Camelia A. Abdel Malak | Hany E. Marei | Mohammad Omran | Asmaa A. Moeit
Abstract: The aim of the present study was to obtain a predominantly pure population of cholinergic neurons from human olfactory bulb neural stem cells (hOBNSCs) using diffusible ligands and to study the gene expression profile for the key genes involved in this transformation (CHAT, CHAT1, NF-L, IIITUB, MAP2, NGFR). Differentiation of hOBNSCs, which was initiated by of BMP9, significantly increased expression of these genes, with highest expression in NGFR followed by CHAT, CHAT1, MAP2, NF-L, and lowest expression in IIITUB. These data provide, for the first time, the ability of hOBNSCs to differentiate in vitro into cholinergic neurons expressing CHAT, MAP2, NF-L, IIITUB, CHAT1, NGFR genes and this can help us to know the potential mechanism of cholinergic differentiation of hOBNSCs which will be helpful when these cells used in treatment of neurodegenerative diseases.
Keywords: human olfactory bulb, neural stem cells, cholinergic neurons, gene expression
Edition: Volume 6 Issue 6, June 2017,
Pages: 751 - 754
Similar Articles with Keyword 'human'
Downloads: 123 | Weekly Hits: ⮙1 | Monthly Hits: ⮙1
Research Paper, Biochemistry Science, India, Volume 7 Issue 4, April 2018Pages: 1397 - 1401
A Study of Biochemical Constituents of Panchakavya
V. Ramya  | S. Karpagam 
Downloads: 14 | Weekly Hits: ⮙1 | Monthly Hits: ⮙2
Informative Article, Biochemistry Science, India, Volume 10 Issue 8, August 2021Pages: 1081 - 1085
Chemistry of Virus and Antiviral Drug Development of Viral Diseases with Chemical Perspective taking COVID-19 Virus as Special Case