Downloads: 114 | Views: 120
Research Paper | Biotechnology | Indonesia | Volume 6 Issue 5, May 2017
Content of Ajmalicine on Cultured Callus Catharanthus roseus (L.) G. Don with Tryptophan Treatment
Stenly J.M. Mandagi | Dingse Pandiangan  | Wenny Tilaar 
Abstract: Catharanthus roseus (L. ) G. Don is cultivated as an ornamental plant and also has a high economic value because it contains terpenoid indole alkaloids such as ajmalicine that have pharmacological benefits. This research was conducted to investigate the content of ajmalicine in callus culture that was given tryptophan treatment with concentrations of 50 mg/L, 100 mg/L, 150 mg/L, 200 mg/L and 250 mg/L. Identification and analysis of ajmalicine using HPLC Shimpak VP-ODS C18 150 x 4.6 mm at a wavelength of 254 nm. The results showed that the ajmalicine content was lower after the tryptophan treatment, where the minimum ajmalicine content of the tryptophan 200 mg/L, ie 0.346 g/g dw. Ajmalicine content of the treatment of 250 mg / L tryptophan, is the highest content in all treatments, ie 2.686 g/g bk. So it can be concluded that the treatment of tryptophan in callus culture Catharanthus roseus can decrease the content of ajmalicine.
Keywords: Ajmalicine, Tryptophan, Callus culture, Catharanthus roseus L G Don
Edition: Volume 6 Issue 5, May 2017,
Pages: 1919 - 1922
Similar Articles with Keyword 'Callus culture'
Downloads: 1 | Monthly Hits: ⮙1
Research Paper, Biotechnology, India, Volume 11 Issue 5, May 2022Pages: 186 - 190
Plant Tissue Culture of Catharanthus roseus or Madagascar Periwinkle as a Continuous Source for the Increased Production of Alkaloids
Stuti Bhatnagar | Rashi Srivastava | Rajesh Saxena
Research Paper, Biotechnology, India, Volume 6 Issue 5, May 2017Pages: 1060 - 1063
Study of in-vitro Micro-propagation of Medicinally Important Plant Andrographis paniculata from its Different Parts
Singh Abhilasha | Awasthi Dr. Arpita