Research Paper | Gynaecology | India | Volume 5 Issue 10, October 2016
Follicular Fluid IL-6 Levels in Prediction of Successful Pregnancy Outcome in Women Undergoing IVF-ET Cycles - A Prospective Study
Yogesh Kumar, Saumya Prasad, Syed Akhtar Husain, Ritu Goyal, Mohammad Aasif Khan, Shashi Sharma, Sudha Prasad
Objective To investigate expediency of quantitative levels of interleukin-6 (IL-6) detected in follicular fluid (FF) of women underwent in-vitro fertilization-embryo transfer (IVF-ET) cycles. Design Prospective observational study. Method Study comprises evaluation of IVF/ICSI-ET cycles in relation to contribution of quantitative levels of IL-6. FF samples were collected on day of oocyte retrieval by pooling ovarian follicles of size 15mm for each woman (n=168). Enzyme-linked immunosorbent assay (ELISA) technique was applied for measuring quantitative level of IL-6. Demography, cycle characteristics, endometrial thickness, number of oocytes, fertilization rate, grading of embryos and IL-6 levels were compared between pregnant and non-pregnant groups of women. Student T-test, Mann Whitney U-test, Chi-squares test and logistic regression were applied as appropriate. Statistical significant level was calculated at P<0.05. Results The women (n=168) were divided into pregnant (Group-A, n=75) and non-pregnant (group-B, n=93) groups. The median level of IL-6 was found lower in group-A as compared to women in group-B [33.9 pg/mL (0.1-199.8) versus 92.9 pg/mL (0.3-199.8), P<0.001 respectively]. Conclusions Lower concentration of IL-6 in follicular fluid was found associated with positive pregnancy outcome and served as reliable predictive marker of successful pregnancy outcome in women underwent assisted reproduction.
Keywords: Infertile-women, Follicular-fluid, Interleukins, IVF-ET and Pregnancy-outcome
Edition: Volume 5 Issue 10, October 2016
Pages: 1724 - 1732
How to Cite this Article?
Yogesh Kumar, Saumya Prasad, Syed Akhtar Husain, Ritu Goyal, Mohammad Aasif Khan, Shashi Sharma, Sudha Prasad, "Follicular Fluid IL-6 Levels in Prediction of Successful Pregnancy Outcome in Women Undergoing IVF-ET Cycles - A Prospective Study", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=ART20162410, Volume 5 Issue 10, October 2016, 1724 - 1732