International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Since Year 2012 | Open Access | Double Blind Reviewed

ISSN: 2319-7064




Downloads: 118

Research Paper | Microbiology | Morocco | Volume 5 Issue 10, October 2016


Comparison of Three Methods for the Detection of Biofilm Formation by Clinical Isolates of Staphylococcus aureus Isolated in Casablanca

Achmit Mohamed | Ait Mhand Rajaa | Zerouali Khalid | Mellouki Fouad | Rhallabi Naima


Abstract: Biofilms are group of microorganisms encased in a matrix of extracellular polysaccharide (slime), called polysaccharide intercellular adhesin (PIA). They have been associated with a variety of chronic and persistent infections. Staphylococcus aureus is from bacteria which have high ability to form a biofilm. In this study, detection of biofilm production by Staphylococcus aureus was done by using three different methods tube method (TM), congo red agar method (CRA) and tissue culture plate method (TCP), three repetitions were made for every method. Among of 117 strains of Staphylococcus spp isolated between October 2015 and January 2016 at the University Hospital Ibn Rochd in Casablanca, 74 were identified as Staphylococcus aureus.58 isolates were detected as biofilm producer by TCP method, 49 by TM and 42 by CRA method. We can conclude from our study that the TCP method is a more quantitative and reliable method for the detection of biofilm formation by Staphylococcus aureus as compared to TM and CRA methods.


Keywords: Biofilm, Staphylococcus aureus, Tube method TM, Congo red agar method CRA, Tissue culture plate method TCP


Edition: Volume 5 Issue 10, October 2016,


Pages: 1156 - 1159


How to Cite this Article?

Achmit Mohamed, Ait Mhand Rajaa, Zerouali Khalid, Mellouki Fouad, Rhallabi Naima, "Comparison of Three Methods for the Detection of Biofilm Formation by Clinical Isolates of Staphylococcus aureus Isolated in Casablanca", International Journal of Science and Research (IJSR), Volume 5 Issue 10, October 2016, pp. 1156-1159, https://www.ijsr.net/get_abstract.php?paper_id=ART20162319

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