International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Since Year 2012 | Open Access | Fully Refereed | Peer Reviewed

ISSN: 2319-7064




Downloads: 127

Research Paper | Veterinary Sciences | India | Volume 5 Issue 4, April 2016


In-vitro Seeding of Bladder Smooth Muscle Cells onto Bladder Acellular Matrix as Cell Delivery Vehicle in Tissue Engineering

Rukmani Dewangan [2] | A. K. Sharma [6] | Naveen Kumar [33]


Abstract: The present study was carried out evaluated efficacy of bladder acellular matrix (BAM) as cell delivery vehicle. Smooth muscle cells were harvested by collagenase digestion of rabbit urinary bladder and the cells were cultured in growth medium comprising of 41 mixture of DMEM and Hams F-12 nutrient mixture supplemented with L-glutamine containing 100 mg/ml penicillin as well as 100 mg/ml streptomycin. Fetal bovine serum was added @ 10 %. The isolated cells were cultured in-vitro to expand into sufficient number for seeding onto xenogenic bladder acellular matrix. Successful in-vitro seeding of bladder smooth muscle cells onto bladder acellular matrix was done.


Keywords: Bladder, Cell culture, Acellular matrix, BAM, Smooth muscle cells


Edition: Volume 5 Issue 4, April 2016,


Pages: 469 - 473


How to Cite this Article?

Rukmani Dewangan, A. K. Sharma, Naveen Kumar, "In-vitro Seeding of Bladder Smooth Muscle Cells onto Bladder Acellular Matrix as Cell Delivery Vehicle in Tissue Engineering", International Journal of Science and Research (IJSR), Volume 5 Issue 4, April 2016, pp. 469-473, https://www.ijsr.net/get_abstract.php?paper_id=5041601

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