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Research Paper | Biochemistry Science | Nigeria | Volume 3 Issue 8, August 2014
Bioethanol Production from Ziziphus abyssinica Agroresidues: a Comparison of Effect of Different Pretreatment Approaches on Amount of Glucose
Samson Baranzan Wayah  | Waheedah Abdallah
Abstract: The aim of this research was to optimize bioethanol production from Ziziphus abyssinica agroresidues (inner stone). Proximate composition of the agroresidues was determined. Seven pretreatment approaches comprising of single-step (Aspergillus niger, sodium hydroxide, sulphuric acid) and multi-step (sodium hydroxide + A. niger, sulphuric acid + A. niger, sulphuric acid + sodium hydroxide, sulphuric acid + sodium hydroxide + A. niger) were carried out. Fermentation was carried out for three (3) days using Saccharomyces cerevisiae. Proximate analysis of these agroresidues revealed that it contain high amount of crude fibre (35.59 %) and non-fibrous carbohydrates (36.60 %) making it potential feedstock for bioethanol production. Pretreatment with dilute sulphuric acid produced the highest amount of glucose (286.80 mg/g biomass) while the unpretreated biomass yielded the lowest amount of glucose (10.87 mg/g biomass). Fermentation under optimized condition yielded bioethanol of concentration 4.5133 % (v/v).
Keywords: Pretreatment, Bioethanol, Ziziphus abyssinica, Agroresidues, Glucose
Edition: Volume 3 Issue 8, August 2014,
Pages: 700 - 704
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