International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Most Trusted Research Journal Since Year 2012

ISSN: 2319-7064



Research Paper | Genetics Science | Egypt | Volume 3 Issue 6, June 2014

Attenuation of Nano-Tio2 Induced Genotoxicity; Mutagenicity and Apoptosis by Chlorophyllin in Mice Cardiac Cells

Hanan Ramadan Hamad Mohamed

Despite uses of titanium dioxide (TiO2) particles in various consumer and medical products therapy increasing human daily exposure; little studies were conducted on its cardiotoxicity. Thus; this study was designed to investigate the possible modulation of nano-TiO2 induced genotoxicity; mutagenicity and apoptosis by chlorophyllin (CHL) coadministration in mice cardiac cells. Mice were injected into the abdominal cavity with TiO2 (500; 1000 and 2000 mg/kg b.w) suspended either in deionized dist. water or in CHL (40 mg/kg b.w) solution for five consecutive days and scarified 24 hour after the last injections. CHL co-administration resulting in significant reductions in tail length; %DNA in tail and tail moment that was highly elevated in nano-TiO2 treated groups in a dose dependent manner; also the appearance of both apoptotic fragmentized Laddered and smeared DNA on agarose gel was returned to the normal un-fragmentized appearance. The observed dose dependent high incidence of mutations inductions in p53 exons (5-8) and myocardial cells infiltrations by inflammatory cells; hemorrhage and congested blood vessels by nano-titanium was attenuated by CHL co-administration. Finally; CHL improved the antioxidant defense system indicated by significant reduction in malondialdiahyde level and increases in reduced glutathione level and glutathione peroxidase activity that were disrupted in nano-titanium treated groups. In conclusion: nano-TiO2 particles induced genotoxicity; mutagenicity and apoptosis were attenuated by CHL co-administration in mice cardiac cells. It is recommended for further studies to detect the CHL dose that completely normalize nano-titanium induced cardiotoxicity.

Keywords: TiO2 nanoparticles, genotoxicity, mutagenicity, p53, apoptosis, mice cardiac cell

Edition: Volume 3 Issue 6, June 2014

Pages: 2625 - 2636


How to Cite this Article?

Hanan Ramadan Hamad Mohamed, "Attenuation of Nano-Tio2 Induced Genotoxicity; Mutagenicity and Apoptosis by Chlorophyllin in Mice Cardiac Cells", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=2014807, Volume 3 Issue 6, June 2014, 2625 - 2636

20 PDF Views | 20 PDF Downloads

Download Article PDF

Top

The material used for high-rise structure i.e. steel, concrete and other. However the whatever type of material used for high-rise structure the main objective of structural engineer remain in safety, minimum material cost, minimum total weight, minimising different sections used while achieving maximum strength of section. In the present work a comparative study is carried out for Indian standard (IS 800-2007) American standard (AISC 13th edition), British standard (BS 5950, 1: 2000) towards achieving these goals.