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Research Paper | Genetics Science | Egypt | Volume 3 Issue 6, June 2014
Comparative Molecular Cytogenetic Analysis of Three Marine Fishes Species of Genus Epinephelus (Family: Serranidae) From Mediterranean Sea
Abu-Almaaty A. H. | Hala El-Sayed Abd-Alaty | Mohamed K. Hassan | Yaseen A. Essa
Abstract: The Metaphase chromosomes of three marine fishes species of genus Epinephelus (Epinephelus aeneus; Epinephelus marginatus and Epinephelus costae (Family: Serranidae) and their karyological and molecular genetic relationships have been studied. All samples were collected from Egyptian Mediterranean Sea Coast. All three species have the same diploid chromosome number of 2n=48. Epinephelus aeneus and Epinephelus costae were identical in their karyotypes (all acrocentric) ; but the karyotype of Epinephelus marginatus was different. Nine of ten RAPD primers; showed polymorphic bands; were used for the construction of the dendrogram and a similarity matrix. A total of 51 bands were obtained; 35 of them were polymorphic. Similarity values among the studied samples ranged from 11 % to 35 %. High similarity values were obtained between Epinephelus aeneus and Epinephelus costae. (35 %) and the low similarity values were obtained between Epinephelus aeneus and Epinephelus marginatus (11 %). The cluster analysis clearly differentiated Epinephelus aeneus and Epinephelus costae from Epinephelus marginatus. RAPD analysis confirmed that the three Epinephelus species under study are genetically different from each other and a genetic variation was found between and within the three species tested in this study.
Keywords: Zoology - Cytogenetics karyotype - RAPD-PCR - DNA Fishes Serranidae Epinephelus - Mediterranean Sea Zoology - Cytogenetics karyotype - RAPD-PCR - DNA Fishes Serranidae Epinephelus - Mediterranean Sea
Edition: Volume 3 Issue 6, June 2014,
Pages: 1121 - 1131