Production; Purification of Pectinase from Bacillus sp. MBRL576 Isolate and its Application in Extraction of Juice
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064



Downloads: 115

Research Paper | Microbiology | India | Volume 3 Issue 6, June 2014

Production; Purification of Pectinase from Bacillus sp. MBRL576 Isolate and its Application in Extraction of Juice

Vibha Bhardwaj, Neelam Garg

A baterium isolated from orchard soil; was selected out of 109 bacterial isolates because of maximum pectinase production at acidic pH and 45o C temperature. The isolate was characterized morphologically; biochemically and culturally and was identified as Bacillus sp. MBRL576 based on nucleotide homology and phylogenetic analysis. Conditions for pectinase production under submerged fermentation from Bacillus sp. MBRL576 were optimized. Enzyme was purified using ion exchange chromatography and SDS PAGE. Crude pectinase was used for extraction and clarification of juice from fruits and vegetable. It was found to increase the clarification and yield of juice.

Keywords: pectin, Bacillus sp MBRL576, pectinase, purification, juice extraction

Edition: Volume 3 Issue 6, June 2014

Pages: 648 - 652

Share this Article

How to Cite this Article?

Vibha Bhardwaj, Neelam Garg, "Production; Purification of Pectinase from Bacillus sp. MBRL576 Isolate and its Application in Extraction of Juice", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=2014323, Volume 3 Issue 6, June 2014, 648 - 652

Enter Your Email Address




Similar Articles with Keyword 'pectin'

Downloads: 103

Research Paper, Microbiology, India, Volume 5 Issue 9, September 2016

Pages: 1428 - 1437

Biochemical, Microbial Stability and Sensory Evaluation of Osmotically Dehydrated Kinnow Peel Candy and Peel Powder

Navneet Sidhu, Maninder Arora, Mohammed Shafiq Alam

Share this Article

Downloads: 109

Research Paper, Microbiology, India, Volume 3 Issue 7, July 2014

Pages: 1091 - 1094

Degradation of Timber Wood by Filamentous Fungi

Ashok K. Shukla

Share this Article

Similar Articles with Keyword 'pectinase'

Downloads: 109

Research Paper, Microbiology, India, Volume 3 Issue 7, July 2014

Pages: 1091 - 1094

Degradation of Timber Wood by Filamentous Fungi

Ashok K. Shukla

Share this Article

Downloads: 129

Research Paper, Microbiology, India, Volume 3 Issue 6, June 2014

Pages: 261 - 265

Pectinase Production by Delftia Acidovorans Isolated From Fruit Waste under Submerged Fermentation

Vibha Bhardwaj, Neelam Garg

Share this Article

Similar Articles with Keyword 'purification'

Downloads: 111

Research Paper, Microbiology, India, Volume 3 Issue 6, June 2014

Pages: 1759 - 1763

Optimization; Purification of Cellulase Produced from Bacillus subtilis subsp. inaquosorum under Solid State Fermentation and its Potential Applications in Denim Industry

Richa Gautam, Jitender Sharma

Share this Article

Downloads: 114

Research Paper, Microbiology, India, Volume 3 Issue 9, September 2014

Pages: 2041 - 2044

Occurance of Keratinophilic Fungi from the Soils of Chhattisgarh

Ashok K. Shukla

Share this Article



Top