International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Most Trusted Research Journal Since Year 2012

ISSN: 2319-7064



Research Paper | Microbiology Science | India | Volume 3 Issue 6, June 2014

Optimization of Production Media of Novel Phytase from Aspergillus Niger Using Wheat Bran Waste

Manish Kumar, Sushma

Phytase; an acid phosphatase enzyme; efficiently cleaves phosphate moieties from phytic acid thereby generating myo-inositol derivatives and inorganic phosphate; was indentified during screening of endophytic fungi Aspergillus niger. Phytase was produced in selective media at room temperature for 3 days. Production media was also optimized varying the concentration of ingredients from 5 to 25g by using solid-state fermentation for 7 days to check the activity of phytase. The zone of inhibition of crude and pure phytase were 2.1 and 2.4cm recorded using well diffusion method.

Keywords: phytase, wheat bran waste, inorganic phosphate, Aspergillus niger, solid state fermentation

Edition: Volume 3 Issue 6, June 2014

Pages: 949 - 951


How to Cite this Article?

Manish Kumar, Sushma, "Optimization of Production Media of Novel Phytase from Aspergillus Niger Using Wheat Bran Waste", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=2014204, Volume 3 Issue 6, June 2014, 949 - 951

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