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Research Paper | Chemistry | India | Volume 3 Issue 5, May 2014
A New Spectrophotometric Method for the Determination of Isoprothiolane in Environmental Samples
Mamta Nirmal | Manish Kumar Rai  | Vindhiya Patel 
Abstract: A new spectrophotometric method for the determination of fungicide isoprothiolane. The method is based on the bromination of isoprothiolane to form dibromoisoprothiolane which react with Potassium iodide-Potassium iodate mixture in the presence of leucocrystal violet (LCV) to form a violet colored complex which is soluble in 30 % alcohol. The complex shows maximum absorbance at 600 nm. Beers law obeyed over the concentration range of 5.0-25 g in a final solution volume of 10 ml. The molar absorptivity of the colored system is 4.6104 L mol-1cm-1 and Sendells sensitivity is 2.010-3 g cm-2. The reproducibility assessed by carrying out seven days replicate analysis of a solution containing 20 g of isoprothiolane in a final solution volume of 10 ml. The standard deviation and relative standard deviation for the absorbance value were found to be 1.3310-4 and 4.110-2 % respectively. The proposed method is free from the interference of other toxicants. The analytical parameters were optimized and the method was applied to the determination of isoprothiolane in water; food and human sample.
Keywords: Spectrophotometery, Isoprothiolane, Bromination, Leucocrystal violet, Violet Dye
Edition: Volume 3 Issue 5, May 2014,
Pages: 1820 - 1822
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