Prevention of Biofilm Formation in Urinary Catheters by treatment with Antibiofilm Agents
International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
www.ijsr.net | Open Access | Fully Refereed | Peer Reviewed International Journal

ISSN: 2319-7064

Research Paper | Microbiology | India | Volume 3 Issue 4, April 2014

Prevention of Biofilm Formation in Urinary Catheters by treatment with Antibiofilm Agents

Niraj A Ghanwate, P V Thakare, P R Bhise, Swapnil Tayde

Urinary Tract Infections (UTI) accounts for an estimated 25-40 % nosocomial infection, out of which 90 % are associated with urinary catheters, called Catheter associated urinary tract infection (CAUTI). The microbial populations within CAUTI frequently develop as biofilms. In the present study, microbial contamination of indwelling urinary catheters was investigated. Biofilm forming ability of the isolates was determined by tissue culture plate method. Prevention of biofilm formation in the urinary catheter by Pseudomonas aeruginosa was also determined by coating catheters each with different antibiofilm agents like DNAse enzyme, antimicrobial drugs- Ceftazidime and Ceftriaxone, anticancer drug-Cisplatin and anticoagulant heparin. It was found that 64 % of the urinary catheters get contaminated during the course of catheterization. Of the total 6 isolates, biofilm formation was seen in 100 % of the Pseudomonas aeruginosa and E. coli isolates 90 % in Enterococci, 80 % in Klebsiella and 66 % in S. aureus. It was noted that the biofilm production by Pseudomonas was prolonged by 14 days in Ceftazidime coated catheter, 8 days in Ceftriaxone and Cisplatin treated catheters, 6 days in heparin and 5 days in DNAse treated catheters.

Keywords: CAUTI, biofilm, enzyme, Ceftazidime, Pseudomonas

Edition: Volume 3 Issue 4, April 2014

Pages: 714 - 717

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How to Cite this Article?

Niraj A Ghanwate, P V Thakare, P R Bhise, Swapnil Tayde, "Prevention of Biofilm Formation in Urinary Catheters by treatment with Antibiofilm Agents", International Journal of Science and Research (IJSR), https://www.ijsr.net/search_index_results_paperid.php?id=20131579, Volume 3 Issue 4, April 2014, 714 - 717

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