International Journal of Science and Research (IJSR)

International Journal of Science and Research (IJSR)
Since Year 2012 | Open Access | Double Blind Reviewed

ISSN: 2319-7064


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Research Paper | Biology | India | Volume 2 Issue 9, September 2013


Caspase Dependent Apoptosis is Only Inhibited on y Irradiation of Cells Conditioned by Repetitive Oxidative Stress

Rita Ghosh [2] | Koyeli Girigoswami [2] | Dipanjan Guha [2]


Abstract: V79C cells were derived from V79 cell line by through chronic oxidative stress that was found to be radio-resistant. These cells had demonstrated transformationlike stable changes and could be used as model system to study oxidant induced carcinogenesis. Our objective was to understand mechanism of radiation-resistance in these cells. Apoptotic cell death was inhibited in these cells as visualized microscopically by Hoechst staining and nucleosomal ladder formation in agarose gel. Release of cytochrome c in cytoplasm and Apoptosis Inducing Factor in the mitochondrial and nuclear fraction of cells were determined by Western blotting. The caspase 9 and caspase 3 activities in these cells were estimated from fluorimetric assays. These results revealed that the radiation resistance was due to inhibition of caspase-dependent apoptotic death pathways although Apoptosis Inducing Factor mediated pathway remained unaffected. These findings may aid in understanding the mechanism of radiation resistance in tumors arising from oxidative stress.


Keywords: gamma rays, Cytochrome c, Caspases, Apoptosis inducing factor


Edition: Volume 2 Issue 9, September 2013,


Pages: 12 - 18


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How to Cite this Article?

Rita Ghosh, Koyeli Girigoswami, Dipanjan Guha, "Caspase Dependent Apoptosis is Only Inhibited on y Irradiation of Cells Conditioned by Repetitive Oxidative Stress", International Journal of Science and Research (IJSR), Volume 2 Issue 9, September 2013, pp. 12-18, https://www.ijsr.net/get_abstract.php?paper_id=02013290

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